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Synonyms
RFP; RNF76
Plasmid
pFB.Dual DAC-TEV (not blunt) TRIM27/6His-C3-UBE20
Parent Plasmid
pFastbacDual DAC TEV
Expressed
DAC-TEV (not blunt) TRIM27
DU Number
DU45791
Genbank
NM_006510.4 GI:115387097 with EST-derived residue change E446K (present in other DU TRIM27 clones)
Tag
DAC
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCAC
CTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCG
GCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAG
ACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACAT
GCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGC
GCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAG
CACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTG
CGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGC
TCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGAC
CATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGA
ACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGA
TTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTAT
CGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCAT
CAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCC
TGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTG
CAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGA
ACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCC
AAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATG
CAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTC
AGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCT
CTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCT
GACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATG
CTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCA
AGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTA
ACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAA
AGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCC
CGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTAAGGTCTCC
TTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTAC
CTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGA
AAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTT
TCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGC
GGCCGC
CTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCG
GCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAG
ACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACAT
GCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGC
GCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAG
CACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTG
CGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGC
TCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGAC
CATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGA
ACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGA
TTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTAT
CGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCAT
CAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCC
TGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTG
CAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGA
ACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCC
AAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATG
CAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTC
AGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCT
CTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCT
GACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATG
CTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCA
AGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTA
ACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAA
AGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCC
CGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTAAGGTCTCC
TTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTAC
CTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGA
AAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTT
TCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGC
GGCCGC
Amino Acid Sequence
View Sequence
MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPENLYFQGGSMASGSVAECLQQETTCPVCLQYFAEPMM
LDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLV
KQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHS
VLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQME
REKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISH
LSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIH
IFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPS
LILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVG
DKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALP
LRTPLQRVGIFLDYDAGKVSFYNVTERCHTFTFSHATFCGPVRPYFSLSY
SGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP*
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPENLYFQGGSMASGSVAECLQQETTCPVCLQYFAEPMM
LDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLV
KQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHS
VLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQME
REKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISH
LSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIH
IFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPS
LILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVG
DKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALP
LRTPLQRVGIFLDYDAGKVSFYNVTERCHTFTFSHATFCGPVRPYFSLSY
SGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
6His-C3-UBE20 from DU45751 cloned NsiI/NsiI into DU45714 at NsiI site.
100042 R 1000 1001 1047 19007
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