ARIH1 | Ubiquitylation Resource

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Synonyms

ARI, HARI, HHARI, UBCH7BP

Plasmid

pET15 6HIS SUMO ARIH1 T177-N393 C357A (ecoli optimised)

Parent Plasmid

pET15b 6His SUMO1

Expressed

6His-SUMO-ARIH1 T177-N393 C357A (ecoli optimised)

DU Number

DU24103

Genbank

E coli optimized gene

Tag

6His

SUMO

Species

Human

Sequence of Insert

View Sequence

ACCCGTAGCAGCGCACAGGATATGCCGTGTCAGATTTGTTATCTGAATTA
TCCGAACAGCTATTTCACCGGTCTGGAATGTGGTCACAAATTTTGTATGC
AGTGTTGGAGCGAATATCTGACCACCAAAATCATGGAAGAAGGTATGGGT
CAGACCATTAGCTGTCCGGCACATGGTTGTGATATTCTGGTTGATGATAA
TACCGTGATGCGTCTGATTACCGATAGCAAAGTGAAACTGAAATATCAGC
ATCTGATCACCAACAGCTTCGTGGAATGTAATCGTCTGCTGAAATGGTGT
CCTGCACCGGATTGTCATCATGTTGTTAAAGTTCAGTATCCGGATGCAAA
ACCGGTTCGTTGTAAATGTGGTCGTCAGTTTTGTTTTAACTGCGGTGAAA
ATTGGCATGACCCTGTTAAATGTAAATGGCTGAAAAAATGGATCAAAAAA
TGCGACGATGATAGCGAAACCAGCAATTGGATTGCAGCAAATACCAAAGA
ATGTCCGAAATGTCATGTGACCATTGAAAAAGATGGTGGCgcCAATCATA
TGGTTTGCCGTAATCAGAATTGCAAAGCCGAATTTTGTTGGGTTTGTCTG
GGTCCGTGGGAACCGCATGGTAGCGCATGGTATAATTGTAATCGCTATAA
Ttaagcggccgc

Amino Acid Sequence

View Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIME
EGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRL
LKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKK
WIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGANHMVCRNQNCKAEFC
WVCLGPWEPHGSAWYNCNRYN*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Linker sequence deleted after SENP cleavage silent mutation t270c compared to NCBI ref
Parental Plasmid DU32993

100042 R 1000 1001 1047 19007

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