This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Synonyms
MTMR15
Plasmid
pBabe.puro.FLAG.DU mSLX4 H109-M184-mFAN1 G68-G1020(end)
Parent Plasmid
pEGFPN1
Expressed
FLAG-mSLX4 H109-M184-mFAN1 G68-G1020(end)
DU Number
DU45709
Genbank
FAN1: NM_177893.3 a414g silent. SLX4: NM_177472.4 2
Tag
FLAG
Species
Mouse
Sequence of Insert
View Sequence
GCGGCCGCGCATGACAGCCTGGAGGAGAAGGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATGTAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAGGCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAAACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGGTGAGGATGGGTGTTGGTGATGACGTTCAAGTAGAGCCTGCTCAGGCTGGATTAATGAGTCCAACTGTGCCTACATCAGATTTACCCAGTGGTCCTTTAGAGAATGTGACACCTCAGAAATTATCACCACCAAAGAGAAGTTTAATTTCTGTCCAATGTGGTTCAAAACTGGGCATACAACAGCAGACCAGTCCCTACTTTAAAGATGCCTTAGTGTCCAAGGATCAAAATGAGCTTCCAAATCAGAGTGTAGAAATTATGCCTTTGGGAAGTCTGACATCGAAATTGTCCAGACGATACTTAAACGCTAAAAAATCACTCGCTAAGAATGAGGGACTAGCCAGTCAGTGCCCACAGACTTCTCCGTCCACACCTGGCACCAGCCTGACTGATAACTGTCCAGAGATGGAAGACAAAGATGAGGTTTTGAACAGTTCCCAAAAGGAAAACATTTATTCATGTGCACCTCTAAAAGAAGAGAATGCTTCAGAACAGAAGGTAAAGAACAATAAAATAACGGGAGATGAAAGCCAGAAAGCTTCCTGCGGGGAGCCAGCCCTCACCCCTGCATCTGCAGAGCATGCTTCCATATTACTGTCCTCAGATTCAACTCTCGTCAGTAACACGAAATCCTCTCCGGGAGACACTCTTGTAAAGCAAGAAAGTGCCAGAAGGGCAGATGTAGGGCTTGCTGAGCCGCTCGAGGTGCGCAGTCACAAAGAAGTACAAATGACTTTCGATGCTGCGGCCAAAACGCTGGTGTCTGGGGAGGCAGAATCAAATGGTCCTACCGATGTTGACATGTCTGACATGACTACGTGGAGTAACAACCAAGAGCTTGTCAGGGAAGCTGGCAGTGTCTTACACTGCCCTCTGGAGCAGGGGTCCAGCTGCGGTGGCCCCAGTGAGACAGCTCAACTGGCACTGAGTCACCCGTACTACCTGCGGAGCTTCCTGGTGGTGCTGCAGGCCCTCCTTGGGAACGAAGAGGACATGAAGCTCTTCGATGAGCAGGAGAAGGCGATTATCACCAGGTTTTACCAGTTGTCAGCTAGTGGTCAAAAGTTGTATGTGAGGCTCTTTCAACGTAAATTAACCTGGATTAAAATGAGTAAACTGGAGTATGAAGAGATTGCCTCCGACTTAACCCCTGTGGTTGAAGAATTGAAGGACTCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGATGTACTTGAACTCCTTTCTGCTCCTGAGCTGAAAGCCCTGGCCAAAACCTTCCACTTGGTGAGTCCCGGTGGGCAGAAGCAGCAGCTGGTAGACGCCTTTCACAAACTGGCCAAACAGCGCTCAGTCTGCACGTGGGGCAAGACTCAGCCTGGAATCCGAGCAGTGATTTTAAAAAGAGCCAAAGACTTGGCTGGCCGGTCGCTTCGAGTCTGTAAAGGCCCTAGGGCTGTGTTTGCCCGAATCTTGCTTCTGTTCTCATTGACTGATTCCATGGAAGACGAAGAAGCTGCTTGTGGGGGTCAAGGTCAGCTGTCTACTGTGCTATTGGTCAATCTGGGCCGAATGGAGTTTCCTCAGTACACCATCTGCCGGAAGACCCAGATCTTCAGGGACAGAGAGGACCTCATCAGATACGCAGCAGCCGCACACATGCTGAGTGACATCTCAGCTGCCATGGCCAGTGGGAACTGGGAGGACGCTAAGGAGCTTGCTCGGAGTGCAAAAAGGGACTGGGAACAACTGAAAAGCCACCCTTCCCTGAGGTACCACGAGGCCTTGCCGCCCTTCCTGCGCTGCTTTACCGTCGGGTGGATCTACACAAGGATTTCCTCTCGGGCTGTTGAAGTCCTAGAGAGGCTTCACATGTACGAGGAAGCTGTCAAGGAACTTGAAAACCTCTTGTCTCAGAAAATCTATTGTCCTGACAGCAGAGGCCGCTGGTGGGATCGCCTGGCTCTTAACCTGCACCAGCACCTGAAGCGTCTGGAAGAGGCAATTAGGTGCATCAGGGAAGGCCTGGCAGACCCACACGTGAGGACAGGACACCGACTTTCTCTTTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGCAGGAAGTACAAGCACCTCTTCAGCCGGCTGCCCGAGGTTGCCGTGGGAGACGTCAAGCATGTGACCATCACGGGCAGGCTGTGCCCACAGCATGGCATGGGCAAGTCTGTGTTTGTCATGGAGAGTGGAGACGGCGCCAACCCCACCACAGTCCTGTGTTCTGTGGAGGAGCTGGCCCTGGGTTATTACAGGCAGAGTGGCTTTGACCAAGGGATTCATGGAGAAGGGTCCACCTTCAGCACCCTGTGTGGCCTCCTGCTGTGGGATATCATCTTCATGGATGGAATACCAGACGTCTTCAGAAATGCCTACCAGGCATCCCCACTGGATTTGCTCACGGACAGCTTCTTCGCAAGCAGGGAGCAGGCCCTGGAAGCCAGGCTGCAGCTGATCCACAGCGCCCCTGCTGAGAGCCTGCGGGCCTGGGTGGGCGAAGCATGGCAGGCCCAGCAAGGCAGAGTGGCCTCTCTGGTCAGCTGGGACCGCTTCACCTCCCTACAGCAAGCTCAGGATCTTGTCTCCTGCCTCGGGGGTCCTGTCCTCAGTGGTGTGTGCAGGCGCCTGGCTGCTGACTTTCGGCACTGCCGAGGGGGCCTCCCAGACTTGGTGGTGTGGAATTCTCAGAGCCACCATTGCAAGCTGGTGGAGGTGAAAGGCCCCAGTGATCGACTTTCATGTAAGCAGATGATCTGGCTGTACGAACTCCAAAAGCTGGGGGCTGATGTAGAAGTGTGCCATGTGGTTGCAGTTGGTGCTAAGAGCAAAGGTCTTGGCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSSEFEVDSLESAAAHDSLEEKGLFFCQMCQKNLSAMNVTR
REQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRM
GVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQC
GSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNA
KKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYS
CAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDS
TLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTL
VSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSE
TAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASG
QKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQE
LSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGK
TQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAA
CGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSD
ISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGW
IYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALN
LHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKH
LFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSV
EELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQ
ASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVA
SLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVW
NSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSK
GLG*
REQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRM
GVGDDVQVEPAQAGLMSPTVPTSDLPSGPLENVTPQKLSPPKRSLISVQC
GSKLGIQQQTSPYFKDALVSKDQNELPNQSVEIMPLGSLTSKLSRRYLNA
KKSLAKNEGLASQCPQTSPSTPGTSLTDNCPEMEDKDEVLNSSQKENIYS
CAPLKEENASEQKVKNNKITGDESQKASCGEPALTPASAEHASILLSSDS
TLVSNTKSSPGDTLVKQESARRADVGLAEPLEVRSHKEVQMTFDAAAKTL
VSGEAESNGPTDVDMSDMTTWSNNQELVREAGSVLHCPLEQGSSCGGPSE
TAQLALSHPYYLRSFLVVLQALLGNEEDMKLFDEQEKAIITRFYQLSASG
QKLYVRLFQRKLTWIKMSKLEYEEIASDLTPVVEELKDSGFLQTESELQE
LSDVLELLSAPELKALAKTFHLVSPGGQKQQLVDAFHKLAKQRSVCTWGK
TQPGIRAVILKRAKDLAGRSLRVCKGPRAVFARILLLFSLTDSMEDEEAA
CGGQGQLSTVLLVNLGRMEFPQYTICRKTQIFRDREDLIRYAAAAHMLSD
ISAAMASGNWEDAKELARSAKRDWEQLKSHPSLRYHEALPPFLRCFTVGW
IYTRISSRAVEVLERLHMYEEAVKELENLLSQKIYCPDSRGRWWDRLALN
LHQHLKRLEEAIRCIREGLADPHVRTGHRLSLYQRAVRLRESPSCRKYKH
LFSRLPEVAVGDVKHVTITGRLCPQHGMGKSVFVMESGDGANPTTVLCSV
EELALGYYRQSGFDQGIHGEGSTFSTLCGLLLWDIIFMDGIPDVFRNAYQ
ASPLDLLTDSFFASREQALEARLQLIHSAPAESLRAWVGEAWQAQQGRVA
SLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRRLAADFRHCRGGLPDLVVW
NSQSHHCKLVEVKGPSDRLSCKQMIWLYELQKLGADVEVCHVVAVGAKSK
GLG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
Download datasheet