HOIP

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Synonyms
RNF31
Plasmid
pGEXTEV HOIP codon opt
Parent Plasmid
pGEX TEV
Expressed
GST TEV HOIP
DU Number
DU43825
Genbank
NP_060469.4
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGCCTGGTGAAGAAGAAGAACGCGCATTTCTGGTTGCACGTGA
AGAACTGGCAAGCGCACTGCGTCGTGATAGCGGTCAGGCATTTAGCCTGG
AACAGCTGCGTCCGCTGCTGGCAAGCAGCCTGCCTCTGGCAGCACGTTAT
CTGCAGCTGGATGCAGCACGTCTGGTTCGTTGTAATGCACATGGTGAACC
GCGTAATTATCTGAATACCCTGAGCACCGCACTGAACATTCTGGAAAAAT
ATGGTCGTAATCTGCTGAGTCCGCAGCGTCCGCGTTATTGGCGTGGTGTG
AAATTTAACAATCCGGTTTTTCGTAGCACCGTTGATGCAGTTCAGGGTGG
TCGTGATGTTCTGCGTCTGTATGGTTATACCGAAGAACAGCCGGATGGTC
TGAGCTTTCCGGAAGGTCAAGAGGAACCGGATGAACATCAGGTTGCCACC
GTTACCCTGGAAGTTCTGCTGCTGCGTACCGAACTGAGCCTGCTGCTGCA
GAATACCCATCCGCGTCAGCAGGCACTGGAACAACTGCTGGAAGATAAAG
TTGAAGATGATATGCTGCAGCTGAGCGAATTTGATCCTCTGCTGCGTGAA
ATTGCACCGGGTCCGCTGACCACCCCGAGCGTTCCGGGTAGTACTCCGGG
TCCGTGTTTTCTGTGTGGTAGCGCACCGGGTACACTGCATTGTCCGAGCT
GTAAACAGGCACTGTGTCCGGCATGTGATCACCTGTTTCATGGTCATCCG
AGCCGTGCACATCATCTGCGTCAGACCCTGCCTGGTGTTCTGCAGGGCAC
CCATCTGAGCCCGAGCCTGCCTGCAAGTGCACAGCCTCGTCCGCAGAGCA
CCAGCCTGCTGGCACTGGGTGATAGCAGCCTGAGCAGCCCGAATCCGGCA
TCAGCACATCTGCCGTGGCATTGTGCAGCCTGTGCAATGCTGAATGAACC
GTGGGCAGTTCTGTGTGTTGCCTGTGATCGTCCGCGTGGTTGTAAAGGTC
TGGGTCTGGGCACCGAAGGTCCGCAGGGTACAGGTGGTCTGGAACCTGAT
CTGGCACGTGGTCGTTGGGCATGTCAGAGCTGTACCTTTGAAAATGAAGC
AGCAGCCGTTCTGTGTAGCATTTGCGAACGTCCGCGTCTGGCCCAGCCTC
CGAGCCTGGTTGTTGATAGCCGTGATGCAGGTATTTGTCTGCAGCCACTG
CAGCAGGGTGATGCACTGCTGGCGAGCGCACAGAGCCAGGTTTGGTATTG
TATTCATTGTACCTTTTGCAATAGCAGTCCGGGTTGGGTTTGTGTTATGT
GTAATCGTACCAGCTCACCGATTCCAGCACAGCATGCACCGCGTCCGTAT
GCGAGCAGCCTGGAAAAAGGTCCGCCTAAACCGGGTCCTCCGCGTCGTCT
GAGTGCACCGCTGCCGAGCAGCTGTGGTGATCCGGAAAAACAGCGTCAGG
ATAAAATGCGTGAAGAAGGTCTGCAACTGGTTAGCATGATTCGTGAAGGT
GAAGCAGCCGGTGCATGTCCGGAAGAAATCTTTAGTGCACTGCAGTATAG
CGGTACGGAAGTTCCTCTGCAATGGCTGCGTAGTGAACTGCCGTATGTTC
TGGAAATGGTTGCCGAACTGGCAGGTCAGCAAGATCCGGGTCTGGGTGCA
TTTAGCTGTCAAGAGGCACGTCGTGCATGGCTGGATCGTCATGGTAATCT
GGATGAAGCAGTTGAAGAATGTGTTCGTACCCGTCGTCGTAAAGTTCAAG
AACTGCAGAGCCTGGGTTTTGGTCCAGAAGAAGGTAGTCTGCAGGCCCTG
TTTCAGCATGGTGGTGATGTTAGCCGTGCCCTGACCGAACTGCAGCGCCA
GCGCCTGGAACCGTTTCGTCAGCGTCTGTGGGATAGTGGTCCGGAACCGA
CCCCGAGCTGGGATGGTCCGGATAAACAGTCACTGGTTCGTCGTCTGCTG
GCAGTTTATGCACTGCCGAGTTGGGGTCGTGCGGAACTGGCACTGTCACT
GCTGCAAGAAACCCCTCGTAATTATGAACTGGGAGATGTTGTTGAAGCGG
TTCGTCATAGCCAGGATCGTGCCTTTCTGCGTCGCCTGCTGGCCCAAGAA
TGTGCAGTTTGTGGTTGGGCACTGCCGCATAATCGTATGCAGGCGCTGAC
CAGCTGTGAATGTACCATTTGTCCGGATTGCTTTCGCCAGCATTTTACCA
TTGCACTGAAAGAAAAACACATCACCGATATGGTTTGTCCTGCATGTGGT
CGTCCGGATCTGACCGATGATACCCAGCTGCTGAGCTATTTTAGCACCCT
GGATATTCAGCTGCGCGAATCACTGGAACCAGATGCATATGCGCTGTTTC
ATAAAAAACTGACCGAAGGTGTTCTGATGCGTGATCCGAAATTTCTGTGG
TGTGCACAGTGTAGCTTTGGCTTTATCTATGAACGTGAACAGCTGGAAGC
AACCTGTCCGCAGTGTCATCAGACCTTTTGTGTTCGTTGCAAACGTCAGT
GGGAGGAACAGCATCGTGGTCGTAGCTGTGAAGATTTTCAGAATTGGAAA
CGTATGAACGATCCGGAATATCAGGCACAGGGTCTGGCAATGTATCTGCA
AGAGAATGGTATTGATTGCCCGAAATGCAAATTTAGCTATGCACTGGCAC
GCGGTGGTTGTATGCATTTTCATTGCACCCAGTGTCGTCATCAGTTTTGT
AGCGGTTGTTATAATGCCTTCTATGCCAAAAACAAATGCCCAGAACCGAA
TTGCCGTGTGAAAAAAAGCCTGCATGGCCATCATCCGCGTGATTGTCTGT
TTTATCTGCGTGATTGGACCGCACTGCGCCTGCAAAAACTGCTGCAGGAT
AATAATGTGATGTTTAATACCGAACCGCCTGCCGGTGCACGTGCGGTTCC
GGGTGGTGGTTGTCGTGTTATTGAACAGAAAGAAGTTCCGAACGGCCTGC
GTGATGAAGCATGTGGTAAAGAAACTCCGGCAGGTTATGCAGGTCTGTGT
CAGGCACATTATAAAGAATATCTGGTGAGCCTGATTAATGCCCATAGCCT
GGACCCTGCAACCCTGTATGAAGTTGAGGAACTGGAAACCGCAACCGAAC
GCTATCTGCATGTGCGTCCGCAGCCGCTGGCAGGCGAAGATCCTCCTGCA
TACCAGGCACGTCTGCTGCAGAAACTGACAGAAGAAGTGCCGCTGGGTCA
GAGCATTCCGCGTCGCCGTAAATAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMPGEEEERAFLVAREELASAL
RRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVRCNAHGEPRNYLNT
LSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRL
YGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQ
QALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVPGSTPGPCFLCG
SAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSL
PASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCV
ACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQSCTFENEAAAVLCS
ICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTFC
NSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPS
SCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEIFSALQYSGTEVPL
QWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEE
CVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFR
QRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPR
NYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNRMQALTSCECTI
CPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRE
SLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCH
QTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQENGIDC
PKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKS
LHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRV
IEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLY
EVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRR
K*
Vector Type
Bacterial
Antibiotic
Amp
Comments
DNA insert is codon optimized for E coli expression
100042 R 1000 1001 1047 19007
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