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Human UBQLN2 ∆Pxx

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Plasmid
pGEX6P-1 HALO TEV UBQLN2 delta 477-540
Parent Plasmid
pGEX6P1 Halo TEV
Expressed
GST-HALO-UBQLN2 ∆Pxx
DU Number
DU43904
Genbank
NM_013444.3
Tag
GST
HALO
Species
Human
Sequence of Insert
ggatccATGGCTGAGAATGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCG
CGGCCCTGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAA
TCATCAAAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTG
CCCGAGAACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTT
CAAATCCCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAA
AAGATCAAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTT
CACCTTGTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCC
TAGCAATGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTA
ACTCCACACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTG
GGAGGACTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTC
TGAGCTCCAGAGCCAGATGCAGCAGCAGCTTATGGCCAGCCCTGAGATGA
TGATCCAAATAATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCC
GATCTGATGAGGCAGCTCATTATGGCTAATCCACAGATGCAGCAATTGAT
TCAGAGAAACCCAGAAATCAGTCACCTGCTCAACAACCCAGACATAATGA
GGCAGACACTCGAAATTGCCAGGAATCCAGCCATGATGCAAGAGATGATG
AGAAATCAAGACCTGGCTCTTAGCAATCTAGAAAGCATCCCAGGTGGCTA
TAATGCTTTACGGCGCATGTACACTGACATTCAAGAGCCGATGCTGAATG
CCGCACAAGAGCAGTTTGGGGGTAATCCATTTGCCTCCGTGGGGAGTAGT
TCCTCCTCTGGGGAAGGTACGCAGCCTTCCCGCACAGAAAATCGCGATCC
ACTACCCAATCCATGGGCACCACCGCCAGCTACCCAGAGTTCTGCAACTA
CCAGCACGACCACAAGCACTGGTAGTGGGTCTGGCAATAGTTCCAGCAAT
GCTACTGGGAACACCGTTGCTGCCGCTAATTATGTCGCCAGCATCTTTAG
TACCCCAGGCATGCAGAGCCTGCTGCAACAGATAACTGAAAACCCCCAGC
TGATTCAGAATATGCTGTCGGCGCCCTACATGAGAAGCATGATGCAGTCG
CTGAGCCAGAATCCAGATTTGGCTGCACAGATGATGCTGAATAGCCCGCT
GTTTACTGCAAATCCTCAGCTGCAGGAGCAGATGCGGCCACAGCTCCCAG
CCTTCCTGCAGCAGATGCAGAATCCAGACACACTATCAGCCATGTCAAAC
CCAAGAGCAATGCAGGCTTTAATGCAGATCCAGCAGGGGCTACAGACATT
AGCCACTGAAGCACCTGGCCTGATTCCGAGCTTCGCTGCACCTAGTGAAA
CCACGAGTCCTACATCAGAATCTGGACCCAACCAGCAGTTCATTCAGCAA
ATGGTGCAGGCCCTGGCTGGAGCAAATGCTCCACAGCTGCCGAATCCAGA
AGTCAGATTTCAGCAACAACTGGAACAGCTCAACGCAATGGGGTTCTTAA
ACCGTGAAGCAAACTTGCAGGCCCTAATAGCAACAGGAGGCGACATCAAT
GCAGCCATTGAAAGGCTGCTGGGCTCCCAGCCATCGTAAgcggccgc
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMAENGESSGPPRP
SRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISK
RFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQST
QPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTN
FSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQ
LIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPG
GYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENR
DPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASI
FSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNS
PLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQ
TLATEAPGLIPSFAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPN
PEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please subclone human UBQLN2 ∆Pxx (deletion of residues 477-540) to the pGEX6P-1-HALO-TEV vector.
Parental: 24005
100042 R 1000 1001 1047 19007
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