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Plasmid
pET28a HALO TEV HUWE1 R1269-K1690
Parent Plasmid
pET28a HALO TEV
Expressed
HALO TEV HUWE1 R1299-K1690
DU Number
DU43883
Genbank
NM_031407.5
Tag
HALO
Sequence of Insert
View Sequence
ggatcccgaggagaagaggatacagggcaagaggaaggtggctcccgccg
ggaacctcaagtcaaccagcaacaactAcaacagctcatggacatgggct
tcacaagggaacatgcaatggaggcactgttgaacaccagcaccatggag
caggccacagagtaccttttaacccaccctcctccaatcatgggaggagt
tgttcgggatctcagcatgtctgaagaggaccagatgatgagagcaattg
ctatgtctctgggacaggatattccaatggatcaaagggcagagtcacct
gaggaagttgcttgccggaaggaggaagaggaacggaaagctcgggaaaa
gcaggaggaggaagaggctaaatgtctagagaagttccaggatgctgacc
cgttggaacaagatgagctccacactttcacagatactatgttgccaggc
tgcttccaccttcttgatgagctgccagacacagtataccgtgtgtgtga
cctgatcatgacagcaatcaaacgtaatggagcagattatcgtgacatga
ttctgaagcaagtagtcaatcaggtgtgggaagctgctgatgtattgatc
aaagctgctcttcccctgacaacaagtgacacaaaaaccgtgtcagagtg
gataagtcagatggccacactgccccaggcctccaatttggctactagaa
tcttgcttttaacgctactttttgaggagttgaagctaccttgtgcttgg
gtggttgaatcaagtggcatccttaatgtcctaatcaaactcttggaagt
ggttcagccctgcctccaggcagccaaggagcagaaggaagtccagaccc
caaagtggatcacaccagtgttgctcctgattgatttctatgaaaagaca
gccatctcctcaaaaaggagagcccagatgactaagtacctgcaatccaa
cagcaacaactggcgctggtttgatgatcgctctgggcgttggtgtagtt
acagtgcaagcaacaatagcactattgattctgcctggaaatctggagag
acaagcgtgcgattcactgcaggccgaagaagatacacggtccaattcac
tacaatggtgcaggttaatgaggaaacagggaaccgacgccctgtgatgc
tgactctcctcagggtacctcggctgaataaataagcggccgc
ggaacctcaagtcaaccagcaacaactAcaacagctcatggacatgggct
tcacaagggaacatgcaatggaggcactgttgaacaccagcaccatggag
caggccacagagtaccttttaacccaccctcctccaatcatgggaggagt
tgttcgggatctcagcatgtctgaagaggaccagatgatgagagcaattg
ctatgtctctgggacaggatattccaatggatcaaagggcagagtcacct
gaggaagttgcttgccggaaggaggaagaggaacggaaagctcgggaaaa
gcaggaggaggaagaggctaaatgtctagagaagttccaggatgctgacc
cgttggaacaagatgagctccacactttcacagatactatgttgccaggc
tgcttccaccttcttgatgagctgccagacacagtataccgtgtgtgtga
cctgatcatgacagcaatcaaacgtaatggagcagattatcgtgacatga
ttctgaagcaagtagtcaatcaggtgtgggaagctgctgatgtattgatc
aaagctgctcttcccctgacaacaagtgacacaaaaaccgtgtcagagtg
gataagtcagatggccacactgccccaggcctccaatttggctactagaa
tcttgcttttaacgctactttttgaggagttgaagctaccttgtgcttgg
gtggttgaatcaagtggcatccttaatgtcctaatcaaactcttggaagt
ggttcagccctgcctccaggcagccaaggagcagaaggaagtccagaccc
caaagtggatcacaccagtgttgctcctgattgatttctatgaaaagaca
gccatctcctcaaaaaggagagcccagatgactaagtacctgcaatccaa
cagcaacaactggcgctggtttgatgatcgctctgggcgttggtgtagtt
acagtgcaagcaacaatagcactattgattctgcctggaaatctggagag
acaagcgtgcgattcactgcaggccgaagaagatacacggtccaattcac
tacaatggtgcaggttaatgaggaaacagggaaccgacgccctgtgatgc
tgactctcctcagggtacctcggctgaataaataagcggccgc
Amino Acid Sequence
View Sequence
MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENL
YFQGGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNT
STMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQR
AESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDT
MLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAA
DVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKL
PCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDF
YEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAW
KSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNK*
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENL
YFQGGSRGEEDTGQEEGGSRREPQVNQQQLQQLMDMGFTREHAMEALLNT
STMEQATEYLLTHPPPIMGGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQR
AESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDT
MLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAA
DVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKL
PCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDF
YEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAW
KSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNK*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Parental Plasmid DU23222
100042 R 1000 1001 1047 19007
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