FANCI (Xenopus)

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Plasmid
pFastBac-FLAG-FANCI (Xenopus) D338G, C781Y, A1082V, V1244A
Parent Plasmid
pFastbac1
Expressed
Flag-FANCI D338G, C781Y, A1082V, V1244A
DU Number
DU24022
Genbank
NM_001171211.1.
Tag
FLAG
Species
Xenopus laevis
Amino Acid Sequence
MDYKDDDDKDQKILSLAAEEQNDGLQSYLQNLKETELSEIITKHAVKGKD
CGALLRGVFKGSPCSHDVAVRRRLAVYRHCIQLVESGDLQREVASEIMGL
LMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKES
LAYGKGELTGEEFKKQLINSLCSSRWDPQSVIFLTSMFRDVSLSAEELQF
VVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQK
QLVQQENSESLDLEDATIPQDQLHHVEGTIILHIVFAIKLDQELGRELLK
YLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDL
QFHQSSKFLQDLVPQPVCVSSILLETVKNSVYGWDHVTQGLVELGFILMD
SFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEIL
EQVLNRVITKAATPVTHFIDLLSDIVVSAPLILQNSSSKVTEAFDHLSFL
PLTTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFSSQIDARKSAVAGFL
LLLRNFKVLGSLSSSQCSQAIGASQIQVDVHMRYNAAANEAFCLEILGSL
RRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDL
LPPLKLEGCITAQGDHIFLQEPLAHLLCCIHHCLQWYKSSLQQHRNPDED
DDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKN
NIYAVLVMGICEVLIEYNFIIANFSKSKFEDILGLFKCYSKLSDILKEKA
AKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFM
RYSVCVALQKIQQLEETGVTDGPDGQNSEKMFRSLCEITRVLMWRYTSIP
AAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGD
EDEEGSREINVTEKAAFQIKQFQRSLINQLSGGEDDFNSKEALLLVSILS
TLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFK
SPVSVLRELCQDIHGHLGDIDQDIEVEKQSHFATVSLKTVAPTVTLLVLG
QAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGT
LLTACHELVQTALPAGSCTDTLLKELAKMYTILTSVVKYYLQICSSHGGQ
ISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEAL
TATSAKILRDTKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQYMKLSTSRD
FRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Clone has D338G, C781Y, A1082V, V1244A substitutions compared to NCBI ref. Also harbours silent mutation c3753t (R1251)

All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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