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Plasmid
pFBDM-Dac-TEV-Cullin3/FLAG-KLHL15
Parent Plasmid
pFBDM
Expressed
Dac-TEV-Cullin3
DU Number
DU42657
Tag
DAC
Species
Human
Sequence of Insert
View Sequence
ATGTCTAACCTGTCTAAGGGCACTGGCAGCCGTAAGGATACCAAGATGAGAATCAGAGCCTTCCCGATGACTATGGACGAAAAGTACGTGAACTCCATCTGGGACCTGCTCAAGAACGCTATCCAGGAGATCCAAAGGAAGAACAACTCCGGCTTGTCTTTCGAGGAACTGTACAGAAACGCCTACACTATGGTGCTGCACAAGCACGGCGAGAAGCTCTACACTGGCTTGCGTGAAGTGGTCACAGAGCACCTCATCAACAAGGTTCGCGAAGATGTGCTCAACTCTTTGAACAACAACTTCCTGCAGACTCTCAACCAGGCTTGGAACGACCACCAGACAGCTATGGTCATGATCAGGGATATCCTGATGTACATGGACAGAGTCTACGTTCAGCAAAACAACGTGGAGAACGTCTACAACCTCGGATTGATCATCTTCCGTGACCAGGTTGTGCGCTACGGTTGCATCCGTGATCACCTCCGCCAAACCTTGCTGGACATGATCGCTCGTGAACGCAAGGGAGAGGTCGTTGATAGGGGTGCTATCAGAAACGCCTGTCAGATGCTGATGATCCTGGGTCTCGAAGGCAGGAGCGTCTACGAGGAAGACTTCGAGGCTCCCTTCCTCGAAATGTCGGCCGAGTTCTTCCAGATGGAATCCCAAAAGTTCTTGGCTGAGAACAGCGCCTCAGTCTACATCAAGAAGGTTGAAGCCCGTATCAACGAGGAAATCGAGCGCGTTATGCACTGCCTGGACAAGTCAACCGAGGAACCTATCGTCAAGGTGGTCGAGCGCGAACTCATCTCCAAGCACATGAAGACCATCGTCGAGATGGAAAACTCTGGTCTGGTTCACATGCTCAAGAACGGCAAGACTGAGGACCTGGGATGTATGTACAAGCTCTTCTCCAGGGTGCCCAACGGCCTGAAGACTATGTGCGAATGTATGTCCTCTTACCTCAGAGAGCAGGGAAAGGCTTTGGTTTCTGAGGAAGGCGAGGGAAAGAACCCTGTGGACTACATCCAAGGCCTCTTGGATTTGAAGTCTAGGTTCGACCGTTTCCTGCTGGAGTCCTTCAACAACGATAGGCTGTTCAAGCAGACCATCGCCGGAGACTTCGAGTACTTCTTGAACCTGAACTCCCGTTCCCCTGAATACCTCAGCTTGTTCATCGACGATAAGCTGAAGAAGGGAGTGAAGGGTCTCACCGAGCAAGAAGTCGAGACTATCTTGGATAAGGCTATGGTCCTGTTCCGTTTCATGCAGGAAAAGGACGTTTTCGAGCGCTACTACAAGCAACACCTGGCCCGTCGCTTGCTGACTAACAAGTCTGTGAGCGACGATAGCGAAAAGAACATGATCTCAAAGTTGAAGACCGAGTGCGGTTGTCAGTTCACTTCTAAGCTGGAAGGCATGTTCCGTGATATGTCAATCTCGAACACCACTATGGACGAGTTCCGCCAGCACCTGCAAGCTACCGGTGTGTCACTCGGTGGCGTGGACCTGACTGTGCGTGTCCTGACAACCGGCTACTGGCCCACTCAGTCGGCTACACCTAAGTGCAACATCCCCCCTGCTCCACGTCACGCCTTCGAAATCTTCAGGAGATTCTACTTGGCCAAGCACAGCGGACGCCAGCTGACACTCCAACACCACATGGGTTCAGCTGATCTGAACGCCACCTTCTACGGCCCGGTGAAGAAGGAAGACGGCTCCGAGGTTGGAGTGGGAGGTGCTCAGGTGACCGGTTCAAACACTCGCAAGCACATCCTGCAGGTCTCGACATTCCAAATGACCATCTTGATGCTGTTCAACAACAGGGAGAAGTACACATTCGAGGAAATCCAGCAAGAAACCGACATCCCAGAACGTGAGCTGGTGCGTGCTCTGCAGTCGCTGGCTTGCGGAAAGCCAACTCAACGCGTCTTGACAAAGGAGCCGAAGTCCAAGGAAATCGAGAACGGTCACATCTTCACAGTGAACGATCAGTTCACCTCTAAGCTGCACCGTGTTAAGATCCAGACTGTGGCTGCCAAGCAAGGCGAGAGCGACCCCGAAAGGAAGGAGACAAGACAGAAGGTGGACGATGACCGCAAGCACGAAATCGAGGCTGCCATCGTCAGGATCATGAAGAGCAGAAAGAAGATGCAACACAACGTCCTCGTTGCTGAGGTCACCCAGCAACTCAAGGCCAGGTTCTTGCCATCACCGGTTGTGATCAAGAAGAGAATCGAGGGTCTGATCGAGAGAGAATACTTGGCTAGGACTCCAGAGGACAGAAAGGTTTACACTTACGTTGCTTAA
Amino Acid Sequence
View Sequence
MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
EcoRI/NotI cuts out DacTEVCullin3, BamHI/NotI cuts out just Cullin3
100042 R 1000 1001 1047 19007
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