USP9X

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Synonyms
DFFRX, FAF, FAM
Plasmid
pcDNA5 FRT/TO GFPTAP USP9X
Parent Plasmid
pcDNA5 FRT/TO GFPTAP
Expressed
GFP-TAP USP9X
DU Number
DU45479
Genbank
NM_001039591.2 GI:145309310 with silent changes: t856c, g6150a and a7062g. And coding changes a4904g (D1635G) and a7040g (E2347G); EST-derived.
Tag
GFP
TAP
Species
Human
Sequence of Insert
GGATCCCCGGAATTCGCCCTTATGACAGCCACGACTCGTGGCTCTCCGGTCGGAGGGAATGACAACCAGGGCCAGGCTCCTGATGGACAGTCTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCTTCCAATGAAAATTCCCCGGCAACTCCCCCAGATGAGCAAGGTCAAGGTGATGCCCCACCACAGCTTGAAGATGAGGAACCTGCATTTCCACATACTGACTTGGCCAAGTTGGATGACATGATCAACAGGCCTCGATGGGTGGTTCCAGTTTTGCCGAAAGGGGAATTAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAAAAGGGCCTTGATGTTAAAAGTGAAGCATGTCAGCGATTTTTCCGTGATGGGCTAACAATATCATTCACTAAAATTCTTACAGATGAAGCAGTGAGTGGCTGGAAGTTTGAAATTCATAGGTGTATTATTAACAATACTCATCGTCTGGTGGAGCTATGTGTGGCCAAGTTGTCCCAAGACTGGTTTCCACTTTTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTACAATGGTACACGTCCATGTGAATCAGTTTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGTTCTCCAGATCCTCGATCACCAAAGGGTTGGCTAGTGGATCTTCTCAACAAATTTGGCACTTTAAATGGGTTCCAGATTTTGCATGATCGTTTTATTAATGGATCAGCATTAAACGTTCAAATAATTGCAGCCCTTATTAAACCATTTGGGCAATGCTATGAGTTTCTCACTCTTCATACAGTGAAAAAGTACTTTCTTCCAATAATAGAAATGGTTCCACAGTTTCTAGAAAACTTAACTGATGAAGAACTGAAAAAAGAAGCAAAGAATGAAGCCAAAAATGATGCTCTTTCAATGATTATTAAATCTTTGAAGAATTTAGCTTCAAGGGTTCCAGGACAAGAAGAAACTGTTAAAAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTATTGCAAATTTCTTCTTTCAATGGAAAGATGAATGCACTGAATGAAGTTAATAAGGTGATATCTAGTGTATCATACTATACTCATCGACATGGTAATCCTGAGGAGGAAGAGTGGCTCACAGCTGAACGAATGGCAGAATGGATACAGCAGAACAATATCTTATCCATAGTGTTGCGAGATAGTCTTCATCAGCCACAGTATGTAGAAAAGTTAGAGAAGATTCTTCGTTTTGTCATCAAAGAAAAAGCTCTGACCTTACAGGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAGCCATTGTGAAGAATGTACATGATCTCCTGGCAAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCATCTTTTTGATTGTTTTAAGGCCAGTTGGACAAATGCGAGTAAAAAGCAACGTGAAAAGCTACTTGAGCTGATACGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACACAAAGTGTTGAACCTTCTGTGGAATCTGGCTCACAGTGATGATGTGCCTGTAGATATCATGGACCTGGCTCTCAGTGCCCACATAAAAATACTAGATTACAGTTGCTCCCAGGACCGTGATACACAAAAGATCCAATGGATAGATCGCTTTATAGAAGAACTTCGCACAAATGACAAATGGGTTATTCCCGCACTGAAACAAATTAGAGAAATTTGTAGTTTGTTTGGTGAAGCGCCTCAAAATTTGAGTCAAACTCAGCGAAGTCCCCATGTGTTTTATCGCCATGACTTAATCAATCAACTTCAACACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGACTATATGCTAGAGACCATGAAGATTATGACCCACAAACTGTGAGGCTGGGAAGTAGATATAGTCATGTTCAAGAAGTTCAAGAACGGCTTAACTTCCTTAGATTTTTATTGAAGGATGGTCAGCTGTGGCTATGTGCTCCTCAGGCAAAACAAATATGGAAATGCTTAGCTGAGAATGCAGTTTACCTTTGTGATCGTGAAGCCTGTTTTAAGTGGTATTCCAAGTTGATGGGGGATGAACCAGACTTAGATCCTGATATTAATAAGGACTTCTTTGAAAGTAATGTGCTTCAGCTTGATCCTTCTCTGTTAACTGAAAATGGAATGAAGTGTTTTGAGCGATTCTTCAAAGCTGTGAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGGAGAGCCTATATGATGGATGACTTGGAGTTAATAGGATTAGATTACCTTTGGAGGGTCGTGATTCAGAGTAATGATGATATTGCCAGCAGAGCTATAGATCTCCTCAAAGAGATATACACGAACCTTGGTCCAAGACTACAAGTCAATCAGGTGGTGATCCATGAAGACTTCATTCAGTCTTGTTTTGATCGTCTGAAGGCTTCCTATGACACATTGTGTGTTTTGGATGGTGACAAAGACAGTGTTAATTGTGCAAGACAGGAAGCTGTTCGAATGGTTCGAGTATTAACTGTTTTAAGGGAATATATAAATGAATGTGACAGTGATTATCATGAGGAAAGAACAATTCTCCCTATGTCGAGAGCATTCCGCGGTAAACACCTCTCTTTTGTAGTTCGATTTCCAAACCAGGGCAGACAGGTTGATGACTTGGAGGTATGGTCTCATACAAATGATACAATTGGTTCAGTACGACGATGTATTCTCAATCGTATTAAAGCCAACGTAGCCCATACAAAAATTGAGCTCTTTGTGGGCGGTGAGCTGATAGATCCTGCAGATGATAGAAAGTTGATTGGACAATTAAACTTAAAAGATAAATCGCTTATTACAGCCAAACTTACACAGATAAGTTCCAATATGCCTTCAAGCCCTGATAGCTCTTCTGATTCCTCCACTGGATCTCCTGGAAACCATGGTAATCATTACAGTGATGGTCCCAATCCAGAAGTGGAAAGCTGTTTGCCTGGAGTGATAATGTCACTGCATCCCAGATACATCTCTTTTCTTTGGCAAGTTGCAGACTTAGGTAGCAGCCTAAATATGCCACCCCTTAGAGATGGAGCAAGAGTACTTATGAAACTTATGCCGCCAGATAGCACAACGATAGAAAAATTAAGAGCTATTTGTTTAGACCATGCCAAACTTGGAGAAAGCAGCCTTAGTCCATCTCTTGACTCACTTTTCTTTGGTCCTTCAGCCTCACAAGTGCTATATCTAACAGAGGTAGTCTATGCCTTGTTAATGCCTGCTGGTGCACCTCTGGCTGATGATTCCTCTGATTTTCAGTTTCACTTCTTGAAAAGTGGTGGCCTACCCCTTGTACTGAGTATGCTAACCAGAAATAACTTCCTACCGAATGCAGATATGGAAACTCGAAGGGGTGCCTACCTCAATGCTCTTAAAATAGCCAAGCTTTTGCTAACTGCCATTGGCTATGGTCATGTTCGAGCTGTGGCAGAAGCTTGTCAGCCAGGTGTAGAAGGTGTGAATCCCATGACACAGATCAACCAAGTTACCCATGATCAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAATCCATCATCCGAGTGCATGCTTAGAAATGTGTCAGTTCGTCTTGCTCAGCAGATATCTGATGAGGCTTCAAGATATATGCCTGATATTTGTGTAATTAGAGCTATACAAAAAATTATCTGGGCATCAGGATGTGGGTCGTTACAGCTAGTATTTAGCCCAAATGAAGAAATCACTAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACTTGGAAGACGAACAGGTTTGCTGTGAAGCATTGGAAGTGATGACCTTATGTTTTGCCTTGATTCCAACAGCCTTAGATGCTCTTAGTAAAGAAAAGGCTTGGCAGACATTCATCATTGACTTACTATTGCACTGTCACAGCAAAACTGTTCGTCAGGTGGCACAGGAGCAGTTCTTTTTAATGTGCACCAGATGTTGCATGGGACACCGGCCTCTACTTTTCTTCATTACTCTACTCTTTACTGTTTTGGGGAGCACAGCAAGAGAGAGAGCTAAACACTCAGGCGACTACTTTACTCTTTTAAGACACCTTCTTAATTACGCTTACAATAGTAATATTAATGTACCCAATGCTGAAGTTCTTCTCAATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAAAAGAACAGGAGAAACGGGTATTGAAGAGACGATCTTAGAGGGCCACCTTGGAGTGACAAAGGAGTTACTGGCCTTTCAAACTTCTGAGAAAAAATTTCATATTGGTTGTGAAAAAGGAGGTGCTAATCTCATTAAAGAATTAATTGATGATTTCATATTTCCTGCATCCAATGTTTACCTACAGTATATGAGAAATGGAGAGCTTCCAGCTGAACAGGCTATTCCGGTCTGTGGTTCACCACCTACAATTAATGCTGGTTTTGAATTACTTGTAGCATTAGCTGTTGGCTGTGTGAGGAATCTCAAACAAATAGTAGATTCTTTGACTGAAATGTATTACATTGGCACAGCAATAACTACTTGTGAAGCACTTACTGAGTGGGAATATCTGCCACCTGTTGGACCCCGCCCACCCAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTAAAACTGAAGGTAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCTGCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACGTACAATTCATGCATAACCGAATGCAGTACAGTATGGAGTATTTTCAGTTTATGAAAAAACTGCTTACATGTAATGGCGTTTACTTAAACCCTCCTCCCGGGCAAGATCACCTGTTGCCTGAAGCAGAAGAAATCACTATGATCAGTATTCAACTTGCTGCTAGATTCCTCTTTACTACAGGATTTCACACAAAGAAAGTAGTCCGTGGCTCTGCCAGTGATTGGTATGATGCATTGTGTATTCTCCTTCGTCACAGCAAGAATGTACGTTTTTGGTTTGCTCATAACGTCCTTTTTAATGTTTCAAATCGCTTCTCCGAATACCTTCTGGAGTGCCCTAGTGCAGAAGTGAGGGGTGCGTTTGCAAAACTTATAGTCTTTATTGCACATTTTTCCTTGCAAGATGGGCCATGTCCTTCACCTTTTGCCTCTCCTGGACCTTCTAGTCAGGCTTATGACAACTTAAGCTTGAGTGATCACTTACTAAGAGCAGTACTAAATCTCTTGAGAAGGGAAGTTTCAGAGCATGGGCGTCATTTACAGCAGTATTTCAACCTGTTTGTAATGTATGCCAATTTAGGTGTGGCAGAGAAGACACAGCTTCTGAAATTGAGTGTACCTGCTACTTTTATGCTTGTGTCTTTAGATGAAGGTCCAGGTCCTCCAATCAAATACCAGTATGCTGAATTAGGCAAATTATACTCAGTAGTGTCACAGCTGATCCGCTGTTGCAATGTCTCTTCAAGAATGCAGTCTTCAATCAATGGTAATCCTCCTCTTCCCAATCCTTTTGGTGATCCTAATTTATCACAACCTATAATGCCAATTCAGCAGAATGTGGCAGACATTTTATTTGTGAGAACAAGTTATGTGAAGAAAATCATTGAAGACTGCAGTAATTCAGAGGAAACCGTCAAATTGCTTCGTTTTTGCTGCTGGGAGAATCCTCAGTTCTCATCTACTGTCCTCAGTGAACTTCTCTGGCAGGTTGCATATTCCTATACCTATGAACTGCGGCCCTATTTGGATCTGCTTTTGCAAATCTTACTGATTGGGGACTCCTGGCAAACTCACAGGATTCATAATGCACTGAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATCCAGCGCTCTAAGAATCACTATCAAAAAAGAGCATACCAGTGTATAAAATGTATGGTAGCTCTATTTAGTAACTGTCCTGTTGCTTACCAAATCCTGCAGGGCAATGGAGATCTTAAAAGAAAGTGGACCTGGGCAGTGGAATGGCTTGGAGATGAACTTGAAAGAAGACCATATACTGGCAATCCTCAGTACACTTACAACAATTGGTCTCCCCCAGTGCAAAGCAATGAAACGTCCAATGGTTATTTCTTGGAGAGATCACATAGTGCTAGGATGACACTTGCAAAAGCTTGTGAACTCTGTCCAGAGGAGGAGCCAGATGACCAAGATGCTCCAGATGAACATGAGTCGCCTCCACCTGAAGATGCCCCATTGTACCCCCATTCACCTGGATCTCAGTATCAACAGAATAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATCACATGAACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCAGTGAAGAAGTATCCCCACCTCAAACCAAGGATCAATGAGCGGCCGC

Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSPEF
ALMTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPP
DEQGQGDAPPQLEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLL
EAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCI
INNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSS
SVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALN
VQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEA
KNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSF
NGKMNALNEVNKVISSVSYYTHRHGNPEEEEWLTAERMAEWIQQNNILSI
VLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKN
VHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDD
KDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQK
IQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFY
RHDLINQLQHNHALVTLVAENLATYMESMRLYARDHEDYDPQTVRLGSRY
SHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACF
KWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVN
CREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYT
NLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSVNCARQEAV
RMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFVVRFPNQGRQ
VDDLEVWSHTNDTIGSVRRCILNRIKANVAHTKIELFVGGELIDPADDRK
LIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSPGNHGNHYSDGP
NPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMP
PDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYAL
LMPAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNFLPNADMETRRGAY
LNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTQINQVTHDQAVVL
QSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWA
SGCGSLQLVFSPNEEITKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFAL
IPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHR
PLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEV
LLNNEIDWLKRIRDDVKRTGETGIEETILEGHLGVTKELLAFQTSEKKFH
IGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPPTI
NAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPV
GPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDD
MSGDEKQDNESNVDPRDDVFGYPQQFEDKPALSKTEGRKEYNIGVLRHLQ
VIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLD
EALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQ
NLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLK
RFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQ
QSEQSESETAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGERNRWY
KFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNA
YILFYERMDTIDQDDELIRYISELAITTRPHQIIMPSAIERSVRKQNVQF
MHNRMQYSMEYFQFMKKLLTCNGVYLNPPPGQDHLLPEAEEITMISIQLA
ARFLFTTGFHTKKVVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSN
RFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYD
NLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLK
LSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSS
INGNPPLPNPFGDPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEE
TVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIGD
SWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNC
PVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQS
NETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPL
YPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSP
PQTKDQ*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Treat carefully. Possibly more stable than equivalent construct DU10786?

All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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