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Cullin3

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Plasmid
pFBDM-GST-TEV-Cullin3/6His-Rbx/FLAG-KLHL13/HA-Fam83G
Parent Plasmid
pFBDM
Expressed
GST-TEV Cullin 3
DU Number
DU42604
Tag
GST
Species
Human
Sequence of Insert
gtcgacatgtcccctatactaggttattggaaaattaagggccttgtgca
acccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatt
tgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattg
ggtttggagtttcccaatcttccttattatattgatggtgatgttaaatt
aacacagtctatggccatcatacgttatatagctgacaagcacaacatgt
tgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcg
gttttggatattagatacggtgtttcgagaattgcatatagtaaagactt
tgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaa
tgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgta
acccatcctgacttcatgttgtatgacgctcttgatgttgttttatacat
ggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaac
gtattgaagctatcccacaaattgataagtacttgaaatccagcaagtat
atagcatggcctttgcagggctggcaagccacgtttggtggtggcgacca
tcctccaaaatcggatggatccGAAAACCTGTATTTTCAGATGAGCAATC
TGAGCAAAGGCACCGGTTCCCGTAAAGACACCAAAATGCGTATCCGTGCG
TTCCCGATGACGATGGACGAAAAATATGTGAACAGCATTTGGGATCTGCT
GAAAAACGCGATTCAGGAAATCCAACGTAAAAACAATTCAGGTCTGTCGT
TTGAAGAACTGTATCGCAATGCCTACACGATGGTGCTGCATAAACACGGC
GAAAAACTGTATACCGGTCTGCGTGAAGTGGTTACGGAACATCTGATTAA
TAAAGTCCGCGAAGACGTGCTGAACAGCCTGAACAATAACTTTCTGCAGA
CCCTGAATCAAGCATGGAACGATCACCAGACGGCTATGGTGATGATTCGT
GACATCCTGATGTATATGGATCGCGTTTACGTCCAGCAAAATAACGTGGA
AAATGTTTATAACCTGGGTCTGATTATCTTCCGTGATCAGGTCGTGCGCT
ACGGCTGCATCCGTGACCATCTGCGCCAAACCCTGCTGGATATGATTGCC
CGTGAACGCAAAGGCGAAGTTGTGGATCGTGGTGCGATCCGCAACGCCTG
TCAGATGCTGATGATTCTGGGCCTGGAAGGTCGTTCAGTGTATGAAGAAG
ATTTCGAAGCGCCGTTTCTGGAAATGAGTGCCGAATTTTTCCAGATGGAA
TCCCAAAAATTTCTGGCAGAAAATAGTGCTTCCGTTTACATCAAAAAAGT
CGAAGCACGTATTAACGAAGAAATCGAACGCGTCATGCACTGCCTGGATA
AAAGCACCGAAGAACCGATTGTTAAAGTGGTTGAACGCGAACTGATCAGT
AAACACATGAAAACCATCGTTGAAATGGAAAATTCCGGCCTGGTCCACAT
GCTGAAAAACGGCAAAACCGAAGACCTGGGTTGTATGTATAAACTGTTTA
GTCGTGTGCCGAATGGTCTGAAAACCATGTGCGAATGTATGAGCAGCTAC
CTGCGCGAACAGGGCAAAGCACTGGTCTCCGAAGAAGGCGAAGGTAAAAA
CCCGGTGGATTATATCCAGGGTCTGCTGGACCTGAAATCACGTTTTGATC
GCTTCCTGCTGGAATCGTTCAATAACGACCGTCTGTTcAAgCAGACCATT
GCGGGCGATTTTGAATATTTCCTGAATCTGAACTCACGCTCGCCGGAATA
CCTGAGTCTGTTTATCGATGACAAACTGAAAAAAGGCGTGAAAGGTCTGA
CCGAACAGGAAGTTGAAACGATTCTGGACAAAGCAATGGTTCTGTTTCGT
TTCATGCAGGAAAAAGATGTCTTCGAACGCTATTACAAACAACATCTGGC
TCGTCGCCTGCTGACCAATAAAAGCGTGTCTGATGACAGCGAGAAAAACA
TGATCTCTAAACTGAAAACCGAATGCGGCTGTCAGTTCACGAGCAAACTG
GAAGGCATGTTTCGTGACATGAGTATTTCCAATACCACGATGGATGAATT
TCGCCAGCACCTGCAAGCGACCGGTGTGTCACTGGGCGGTGTTGATCTGA
CGGTGCGTGTTCTGACCACGGGTTATTGGCCGACCCAGTCGGCAACGCCG
AAATGCAACATCCCGCCGGCACCGCGTCATGCATTTGAAATTTTCCGTCG
CTTTTATCTGGCCAAACACTCAGGCCGCCAGCTGACCCTGCAACATCACA
TGGGTTCGGCAGACCTGAATGCTACGTTCTACGGCCCGGTGAAAAAAGAA
GATGGCAGCGAAGTCGGTGTGGGCGGTGCCCAGGTGACCGGTAGCAACAC
GCGTAAACATATCCTGCAGGTTTCTACCTTCCAAATGACGATTCTGATGC
TGTTTAATAACCGCGAAAAATACACCTTCGAAGAAATCCAGCAAGAAACG
GATATTCCGGAACGTGAACTGGTGCGCGCGCTGCAGAGCCTGGCATGTGG
TAAACCGACCCAACGCGTTCTGACGAAAGAACCGAAATCTAAAGAAATCG
AAAACGGCCATATCTTCACCGTGAACGACCAGTTTACGAGTAAACTGCAC
CGTGTCAAAATCCAGACCGTGGCGGCCAAACAGGGTGAATCCGATCCGGA
ACGTAAAGAAACGCGCCAGAAAGTGGATGACGATCGCAAACATGAAATTG
AAGCAGCTATTGTTCGTATCATGAAAAGCCGTAAGAAAATGCAGCACAAT
GTTCTGGTCGCAGAAGTTACCCAGCAACTGAAAGCTCGTTTTCTGCCGTC
TCCGGTCGTGATTAAAAAACGCATCGAAGGCCTGATTGAACGTGAATACC
TGGCTCGCACCCCGGAAGATCGTAAAGTCTACACCTATGTCGCCTGAgcggccgc
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Knocked out PmeI restriction site (silent change) to enable further subcloning
100042 R 1000 1001 1047 19007
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