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Plasmid
pET28a 6His-Halo-TEV-USP35 C450S
Parent Plasmid
pET28a 6His Halo TEV
Expressed
6His-Halo-TEV-USP35 C450S
DU Number
DU46073
Genbank
Q9P2H5
Tag
6His
HALO
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGT
CAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGC
CGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTC
TACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCT
GCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCG
CGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCC
CGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCC
TGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCG
TGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTG
GCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTG
CCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCG
AGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTC
CTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAA
AGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCG
CCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTT
GTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGAC
TGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATA
TTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAG
TTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTC
TAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGT
ACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTC
CCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTC
GGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGT
TCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATC
AAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCA
GGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGC
TCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAAC
ACATCCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTT
CAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGA
CCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCC
ATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCC
TGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGC
TGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAG
TCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTC
TGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCT
GCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC
GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGAT
GCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTG
CACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATC
ACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAA
GGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAG
AAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAA
AGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGA
AGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGG
AAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCC
GGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGT
TAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACT
GCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGC
CAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCT
GCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGC
TGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGC
AGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTG
CTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCG
ATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTG
TCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAA
GGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCG
AGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAG
GACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCA
GGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACAT
CTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGC
TCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAG
ACTGGTCTTCTAAGCGGCCGC
CAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGC
CGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTC
TACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCT
GCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCG
CGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCC
CGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCC
TGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCG
TGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTG
GCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTG
CCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCG
AGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTC
CTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAA
AGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCG
CCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTT
GTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGAC
TGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATA
TTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAG
TTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTC
TAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGT
ACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTC
CCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTC
GGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGT
TCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATC
AAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCA
GGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGC
TCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAAC
ACATCCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTT
CAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGA
CCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCC
ATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCC
TGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGC
TGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAG
TCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTC
TGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCT
GCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC
GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGAT
GCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTG
CACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATC
ACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAA
GGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAG
AAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAA
AGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGA
AGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGG
AAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCC
GGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGT
TAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACT
GCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGC
CAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCT
GCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGC
TGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGC
AGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTG
CTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCG
ATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTG
TCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAA
GGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCG
AGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAG
GACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCA
GGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACAT
CTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGC
TCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAG
ACTGGTCTTCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQ
CLALLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLR
LLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPG
PAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVE
FLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVV
QHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWII
ALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTF
QHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGF
PDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSD
TGKIGLINLGNTSYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWL
FGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEK
TGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVS
SREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGR
VGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEER
TEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLG
AGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCAS
LQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPL
AGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPE
NQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGS
SRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGR
GFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQ
CLALLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLR
LLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPG
PAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVE
FLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVV
QHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWII
ALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTF
QHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGF
PDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSD
TGKIGLINLGNTSYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWL
FGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEK
TGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVS
SREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGR
VGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEER
TEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLG
AGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCAS
LQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPL
AGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPE
NQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGS
SRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGR
GFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*
Vector Type
Bacterial
Antibiotic
Kan
Comments
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100042 R 1000 1001 1047 19007
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