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Plasmid
pET15b 6HIS TEV ITCH aa483-862
Parent Plasmid
pET15b 6His TEV1
Expressed
6HIS-ITCH aa483-862
DU Number
DU23900
Genbank
NM_031483
Tag
6His
Species
Human
Sequence of Insert
View Sequence
ggatccTATGTTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTG
GTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAA
AAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAA
GATCTGCGAAGACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGA
TTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGT
TGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGC
TTGCAGATAAACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTT
TCGTTTTATTGGCAGATTcATTGCCATGGCTCTGTTCCATGGGAAATTCA
TAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCA
GTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCT
CATCTGGGTTAAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACT
TCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAA
CCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACAT
CAGAATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACAC
AAGCTTTCTTTGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAA
TACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGAT
TGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGA
CCAGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAAT
GAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCC
AGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCT
GCATTGAAAAAGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGT
TTTAATCGCCTGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGA
AAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAg
cggccgc
GTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAA
AAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAA
GATCTGCGAAGACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGA
TTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGT
TGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGC
TTGCAGATAAACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTT
TCGTTTTATTGGCAGATTcATTGCCATGGCTCTGTTCCATGGGAAATTCA
TAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCA
GTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCT
CATCTGGGTTAAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACT
TCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAA
CCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACAT
CAGAATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACAC
AAGCTTTCTTTGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAA
TACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGAT
TGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGA
CCAGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAAT
GAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCC
AGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCT
GCATTGAAAAAGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGT
TTTAATCGCCTGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGA
AAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAg
cggccgc
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGENLYFQGHMLGSYVRDFKAKVQYFRFWCQQLAMPQHI
KITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFF
LLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMA
LFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEE
CDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSR
GVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAI
YRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGS
NGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETE
GFGQE*
KITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFF
LLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMA
LFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEE
CDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSR
GVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAI
YRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGS
NGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETE
GFGQE*
Vector Type
Bacterial
Antibiotic
Amp
Comments
silent mutation compared to NCBI at Phe603
100042 R 1000 1001 1047 19007
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