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Plasmid
pET28a-6His_TEV-HALO-Mud1 aa291-332 tetramer
Parent Plasmid
pET28a 6His TEV Halo
Expressed
6HIS-TEV-Halo-Mud1 aa 291-332 tetramer
DU Number
DU23893
Genbank
Q10256, NP_001018195.1 NM_001018619.1
Tag
6His
HALO
Sequence of Insert
View Sequence
GGATCCGGCGGAGGCCCCACTGACCCCGGTTTGAACTCCAAAATTGCACAGCTAGTCAGTATGGGATTTGATCCATTAGAGGCTGCGCAGGCGTTAGACGCTGCGAATGGAGATTTAGATGTAGCTGCTTCTTTTCTCCTTGGCGGAGGCAGATCT
Amino Acid Sequence
View Sequence
MGSSHHHHHHENLYFQMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTP
VLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDH
VRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRP
IPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEV
EMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV
PKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGS
EIARWLSTLEISGGSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLD
VAASFLL*
VLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDH
VRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRP
IPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEV
EMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV
PKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGS
EIARWLSTLEISGGSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANG
DLDVAASFLLGGGRSPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLD
VAASFLL*
Vector Type
Bacterial
Antibiotic
Kan
Comments
from Schizosaccharomyces pombe. 22723 ligated to 23827 vector to give the tetramer. Grown at 30 degrees
100042 R 1000 1001 1047 19007
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