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IsoT (USP5)

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Synonyms
USP5
Plasmid
pGEX6P2 MCS-Linker2-IsoT UBP domain (V173-T289)
Parent Plasmid
pGEX6P2
Expressed
GST-MCS-Linker2-IsoT UBP domain (V173-T289)
DU Number
DU45360
Genbank
NM_001098536.1 GI:148727330
Tag
GST
Species
Human
Sequence of Insert
GCGGCCGCGGGAGATGACGTTGATGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGACATAAGGCCGC

Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAGDDVDVRQ
VSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFD
GSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEH
LSHFGIDMLKMQKT*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Mutagenesis to kill 3' Not1 so Not1 in MCS is unique.
100042 R 1000 1001 1047 19007
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