MUD1

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Plasmid
pET28a 6His TEVb HALO Mud1 aa291-332 trimer
Parent Plasmid
pET28a 6His TEVb Halo
Expressed
6HIS-TEVb-Halo-Mud1 aa 291-332 trimer
DU Number
DU23827
Genbank
Q10256, NP_001018195.1 NM_001018619.1
Tag
HALO
Sequence of Insert
GGATCCGGCGGAGGCCCCACTGACCCCGGTTTGAACTCCAAAATTGCACAGCTAGTCAGTATGGGATTTGATCCATTAGAGGCTGCGCAGGCGTTAGACGCTGCGAATGGAGATTTAGATGTAGCTGCTTCTTTTCTCCTTGGCGGAGGCAGATCT
Amino Acid Sequence
MGSSHHHHHHENLYFQMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGGSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLLGGGRSGGGPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLLGGGRSPTDPGLNSKIAQLVSMGFDPLEAAQALDAANGDLDVAASFLLZAA
Vector Type
Bacterial
Antibiotic
Kan
Comments
from Schizosaccharomyces pombe. 22723 ligated twice to 22720 vector to give the trimer. Grown at 30 degrees
Parental Plasmid DU23713
100042 R 1000 1001 1047 19007
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