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Synonyms
MTMR15
Plasmid
pBabe.puro.GFP.DU FAN1 (MTMR15) S126A F129A
Parent Plasmid
pBabeD puro GFP
Expressed
GFP FAN1 (MTMR15) S126A F129A
DU Number
DU45258
Genbank
NM_014967.4 with change t3015c (silent)
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
AGATCTATGATGTCAGAAGGGAAACCTCCTGACAAAAAAAGGCCTCGTAGAAGCTTATCAATCAGCAAGAATAAGAAAAAAGCATCTAATTCTATTATTTCGTGTTTTAACAATGCACCACCTGCTAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCGTTCAAGTGGATCCAGGGCAGGTTGGCTTAATAAATTCAAATGTGTCTATGGTAGATTTAACCAGTGTTACCTTAGAAGATGTAACACCTAAGAAGTCACCACCACCAAAGACAAATTTAACCCCTGGCCAAAGTGATTCAGCAAAAAGGGAAGTAAAGCAGAAGATCGCTCCCTACGCTAAAAGTAATGATGTGGTGTGCAAAAATCAAGATGAGCTGAGAAATCGTAGTGTGAAAGTCATTTGTTTGGGAAGCCTAGCATCTAAATTGTCCAGAAAATACGTAAAGGCTAAAAAATCAATAGATAAGGATGAAGAATTTGCCGGTTCTAGTCCACAGAGTTCCAAATCCACAGTTGTTAAGAGCCTGATTGATAACTCTTCAGAAATTGAGGACGAGGATCAAATTTTGGAGAACAGTTCTCAAAAAGAAAACGTGTTTAAATGTGATTCTCTAAAGGAAGAGTGCATTCCTGAACATATGGTAAGAGGAAGTAAAATAATGGAAGCCGAAAGCCAAAAGGCTACCCGGGAATGTGAGAAATCAGCCCTCACCCCTGGATTCTCAGATAATGCGATCATGTTATTCTCACCAGATTTCACTCTTAGGAATACATTAAAGTCTACTTCAGAAGACAGTCTTGTAAAGCAAGAGTGTATCAAAGAAGTGGTTGAAAAACGTGAGGCATGTCATTGTGAAGAAGTAAAAATGACTGTTGCTTCAGAAGCTAAAATACAGCTGTCAGATTCAGAGGCAAAATCTCATAGTTCTGCAGATGATGCTTCTGCATGGAGTAACATCCAAGAGGCTCCTCTGCAGGATGACAGTTGCTTAAACAATGATATCCCTCACAGCATTCCTTTGGAGCAGGGGTCAAGCTGCAATGGTCCTGGTCAAACAACCGGTCATCCTTACTACCTTCGGAGTTTCCTTGTGGTGCTGAAAACCGTACTTGAGAATGAAGATGATATGTTGCTCTTTGATGAGCAGGAGAAGGGAATTGTAACTAAATTTTATCAGTTATCAGCTACTGGTCAGAAGTTATATGTAAGGCTCTTTCAACGTAAATTAAGCTGGATTAAGATGACCAAATTAGAGTATGAAGAGATTGCCTTAGACTTAACACCTGTGATTGAAGAATTGACGAATGCAGGCTTTCTACAGACAGAATCTGAGTTGCAAGAACTCTCTGAAGTGCTTGAACTCCTTTCTGCTCCTGAACTAAAATCCCTAGCCAAGACCTTCCACTTGGTGAATCCCAATGGACAGAAACAGCAGCTGGTGGACGCCTTTCTCAAATTGGCCAAACAGCGTTCAGTCTGCACTTGGGGCAAGAATAAGCCTGGAATTGGTGCAGTGATTTTAAAAAGAGCCAAAGCCTTGGCTGGACAGTCAGTACGAATCTGTAAAGGCCCCAGGGCTGTGTTTTCCCGCATCTTGCTACTGTTTTCGTTGACCGACTCAATGGAAGATGAAGACGCCGCTTGTGGAGGTCAGGGACAGCTTTCAACAGTCCTGTTGGTCAACCTCGGCCGAATGGAGTTTCCTAGTTACACCATCAATCGGAAAACCCACATCTTCCAAGACAGAGATGATCTTATCAGATATGCAGCAGCCACGCACATGCTGAGTGACATTTCTTCCGCAATGGCCAATGGGAACTGGGAAGAAGCTAAGGAGCTCGCTCAGTGTGCAAAAAGGGATTGGAACAGACTGAAAAACCACCCTTCTCTGAGATGCCACGAAGATTTACCACTCTTCCTGCGGTGTTTCACTGTTGGGTGGATTTATACAAGGATTTTGTCTCGGTTTGTGGAAATACTGCAGAGACTTCACATGTATGAGGAAGCCGTCAGAGAACTTGAAAGCCTTTTGTCTCAGAGAATTTATTGTCCTGACAGCAGAGGCCGATGGTGGGATCGACTGGCCCTTAATTTACACCAGCACTTGAAGCGCCTGGAACCGACTATCAAGTGCATCACAGAGGGGCTGGCGGATCCGGAAGTCAGAACGGGACACCGCCTTTCACTGTATCAGCGAGCCGTGCGCCTGCGAGAGTCTCCGAGCTGTAAAAAGTTCAAGCACCTCTTCCAGCAGCTCCCAGAAATGGCTGTGCAAGATGTGAAACACGTGACCATCACAGGCAGGCTGTGCCCACAGCGTGGGATGTGCAAGTCTGTGTTTGTGATGGAGGCCGGGGAGGCCGCTGACCCCACCACGGTCCTGTGCTCTGTGGAGGAGCTGGCACTGGCCCATTACAGACGCAGCGGTTTTGACCAGGGGATTCATGGCGAAGGGTCCACCTTCAGCACCCTGTATGGCCTCCTCCTGTGGGACATCATCTTCATGGATGGGATTCCGGATGTCTTCAGAAACGCCTGTCAGGCATTCCCCCTGGACTTGTGCACAGACAGCTTCTTCACAAGCAGACGCCCAGCCCTTGAGGCCAGGCTGCAGCTGATTCATGATGCCCCCGAGGAGAGCCTGCGGGCCTGGGTGGCAGCCACGTGGCATGAGCAGGAAGGCAGAGTGGCTTCCCTTGTCAGCTGGGATCGCTTCACGTCTCTTCAGCAAGCTCAGGATCTTGTCTCCTGCCTGGGGGGCCCTGTGCTCAGTGGTGTGTGCAGGCACCTGGCTGCTGACTTTCGACACTGTCGAGGGGGCCTCCCCGACCTGGTGGTGTGGAACTCCCAGAGCCGTCACTTTAAGCTGGTGGAAGTTAAAGGCCCCAATGATCGTCTTTCACATAAGCAGATGATCTGGCTGGCTGAACTGCAGAAGCTGGGGGCTGAAGTAGAAGTCTGCCACGTGGTTGCAGTTGGAGCTAAGAGCCAAAGCCTTAGCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMMSEG
KPPDKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLACPVCSKMVPRYD
LNRHLDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPP
KTNLTPGQSDSAKREVKQKIAPYAKSNDVVCKNQDELRNRSVKVICLGSL
ASKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQI
LENSSQKENVFKCDSLKEECIPEHMVRGSKIMEAESQKATRECEKSALTP
GFSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVK
MTVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSI
PLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVT
KFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGF
LQTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAK
QRSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLT
DSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRY
AAATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPL
FLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRG
RWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRE
SPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAA
DPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGI
PDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAAT
WHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCR
GGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCH
VVAVGAKSQSLS*
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMMSEG
KPPDKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLACPVCSKMVPRYD
LNRHLDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPP
KTNLTPGQSDSAKREVKQKIAPYAKSNDVVCKNQDELRNRSVKVICLGSL
ASKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQI
LENSSQKENVFKCDSLKEECIPEHMVRGSKIMEAESQKATRECEKSALTP
GFSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVK
MTVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSI
PLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVT
KFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGF
LQTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAK
QRSVCTWGKNKPGIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLT
DSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRY
AAATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPL
FLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRG
RWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRE
SPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCPQRGMCKSVFVMEAGEAA
DPTTVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGI
PDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAAT
WHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCR
GGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLGAEVEVCH
VVAVGAKSQSLS*
Vector Type
Mammalian
Antibiotic
Amp
Comments
All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
100042 R 1000 1001 1047 19007
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