VCPIP1

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Synonyms
DUBA3, VCIP135
Plasmid
pGEX6P-1 VCPIP1 92-463
Parent Plasmid
pGEX6P1
Expressed
GST N-term
DU Number
DU44357
Genbank
NM_025054.4
Tag
GST
Species
Human
Sequence of Insert
GGATCCGCCATGGACGTAGTGCTACACAACCTGCTGCGGAACGCGCTGCT
CGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGTAAAGGTGATGG
GCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTAT
GGAATGGACAAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCA
GGGCGAACTGTTCGATTGCGCCTTACTGGGTGACCGCGCCTTCCTCATAG
AACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAGC
CTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAG
CCAGGAATGTCTCATTCCAGTGCATGTGGACGGGGATGGACACTGCTTGG
TGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCTTCTGGCATGCC
TTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCA
AGCTCTGTTCCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCA
ATGAGTGTGACCCTCTGTTTGTACCACCTGAGGGTGTTCCCTTGGGCCTG
AGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTAT
TCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCA
CCTTTCTACCTGGGCTCATCCCTGCAGAGAAGTGCACTGGGAAAGATGGT
CATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCCGGTAGAAACCA
TTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTA
TGAATTTGCTTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAG
TACATAAAACTTGAAGAGGATGGTGGTTGTGTTATTGGAGGTGACAGAAG
TTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTCT
TTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAG
TATTTCTACAGAAGGACTGGAGTGATAGGAGTTCAGCCTGAGGAAGTCAC
AGCAGCTGCTAAAAAAGCAGTAATGGATTAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMDVVLHNLLRNALLGVTG
APKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELF
DCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECL
IPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFH
DFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDS
LSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPL
VGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDK
YLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAK
KAVMD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
residues 92-463, precission cleavage site between GST and protein
100042 R 1000 1001 1047 19007
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