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Plasmid
pcDNA3.1 Flag USP35 C450A
Parent Plasmid
pcDNA3.1 hygro FLAG
Expressed
FLAG-USP35 Cys450Ala
DU Number
DU25170
Genbank
Q9P2H5
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGTCAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGCCGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTCTACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCTGCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCGCGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCCCGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCCTGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCGTGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTGGCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTGCCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCGAGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTCCTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAAAGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCGCCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTTGTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGACTGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATATTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAGTTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTCTAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGTACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTCCCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTCGGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGTTCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATCAAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCAGGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGCTCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAACACATGCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTTCAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGACCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCCATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGCTGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAGTCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTCTGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCTGCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTCGCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGATGCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTGCACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATCACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAAGGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAGAAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAAAGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGAAGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGGAAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCCGGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGTTAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACTGCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGCCAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCTGCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGCTGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGCAGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTGCTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCGATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTGTCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAAGGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCGAGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAGGACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCAGGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACATCTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGCTCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAGACTGGTCTTCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Parental Plasmid DU35004
100042 R 1000 1001 1047 19007
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