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RNF146

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Plasmid
pEFIRES-P-eYFP C1-RNF146 a1053t, g1056a, a1062t
Parent Plasmid
pEFIRES puro eYFPC1
Expressed
eYFP-RNF146 a1053t, g1056a, a1062t
DU Number
DU20609
Genbank
NM_030963.2
Tag
YFP
Species
Human
Sequence of Insert

GCGGCCGCATGGCTGGCTGTGGTGAAATTGATCATTCAATAAACATGCTT
CCTACAAACAGGAAAGCGAACGAGTCCTGTTCTAATACTGCACCTTCTTT
AACCGTCCCTGAATGTGCCATTTGTCTGCAAACATGTGTTCATCCAGTCA
GTCTGCCCTGTAAGCACGTTTTCTGCTATCTATGTGTAAAAGGAGCTTCA
TGGCTTGGAAAGCGGTGTGCTCTTTGTCGACAAGAAATTCCCGAGGATTT
CCTTGACAAGCCAACCTTGTTGTCACCAGAAGAACTCAAGGCAGCAAGTA
GAGGAAATGGTGAATATGCATGGTATTATGAAGGAAGAAATGGGTGGTGG
CAGTACGATGAGCGCACTAGTAGAGAGCTGGAAGATGCTTTTTCCAAAGG
TAAAAAGAACACTGAAATGTTAATTGCTGGCTTTCTGTATGTCGCTGATC
TTGAAAACATGGTTCAATATAGGAGAAATGAACATGGACGTCGCAGGAAG
ATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAG
GCTAGACTGTGATGCTAATACCGTAAACCTAGCAAGAGAGAGCTCTGCTG
ACGGAGCGGACAGTGTATCAGCACAGAGTGGAGCTTCTGTTCAGCCCCTA
GTGTCTTCTGTAAGGCCCCTAACATCAGTAGATGGTCAGTTAACAAGCCC
TGCAACACCATCCCCTGATGCAAGCACTTCTCTGGAAGACTCTTTTGCTC
ATTTACAACTCAGTGGAGACAACACAGCTGAAAGGAGTCATAGGGGAGAA
GGAGAAGAAGATCATGAATCACCATCTTCAGGCAGGGTACCAGCACCAGA
CACCTCCATTGAAGAAACTGAATCAGATGCCAGTAGTGATAGTGAGGATG
TATCTGCAGTTGTTGCACAGCACTCCTTGACCCAACAGAGACTTTTGGTT
TCTAATGCAAACCAGACAGTACCCGATCGATCAGATCGATCGGGAACTGA
TCGATCAGTAGCAGGGGGTGGAACAGTGAGTGTCAGTGTCAGATCTAGAA
GGCCTGATGGACAATGCACTGTAACTGAAGTTTAAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSRVDPGGRMAGCGEIDHSINMLPTNRKANESCSNTAP
SLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPE
DFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFS
KGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAG
LRLDCDANTVNLARESSADGADSVSAQSGASVQPLVSSVRPLTSVDGQLT
SPATPSPDASTSLEDSFAHLQLSGDNTAERSHRGEGEEDHESPSSGRVPA
PDTSIEETESDASSDSEDVSAVVAQHSLTQQRLLVSNANQTVPDRSDRSG
TDRSVAGGGTVSVSVRSRRPDGQCTVTEV*



Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression vector for eYFP-RNF146 a1053t, g1056a, a1062t. EF1a promoter, IRES and puromycin selection cassette

Estimated MW of expressed protein : 68804 Daltons
100042 R 1000 1001 1047 19007
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