Cullin3

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Synonyms
CUL3
Plasmid
pcDNA5-FRT/TO-HA Cullin3
Parent Plasmid
pcDNA5 FRT/TO HA1
Expressed
HA-Cullin3
DU Number
DU20902
Genbank
NM_003590
Tag
HA
Species
Human
Sequence of Insert
GGATCCATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGTATCCGGGCCTTTCCGATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACAGATATCCCTGAAAGAGAGCTTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTCATATATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAACAAGGTGAATCCGACCCAGAGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAACATGAGATAGAAGCTGCTATAGTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAATGTTCTAGTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATAAGCGGCCGCT
Amino Acid Sequence
MYPYDVPDYAGSMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVAZAAA
Vector Type
Mammalian
Antibiotic
Amp
Comments
Has silent mutation g51t, KO internal BamH1 site
Mammalian Flp-In plasmid to express Cullin3 with N-terminal HA

Estimated MW of expressed protein : 90291 Daltons
100042 R 1000 1001 1047 19007
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