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Synonyms
secreted effector protein
Plasmid
pGEX TEV NLEL 59-end (E.coli)
Parent Plasmid
pGEX TEV
Expressed
GST TEV NLEL 59-end (E.coli)
DU Number
DU39419
Genbank
NP_309587.1
Tag
GST
Species
Escherichia coli
Sequence of Insert
View Sequence
GGATCCAACGGCGAAACCCTGAGCATTAGCGAACCGATTACCACCCTGCCGGATCTGCTGCCGGGCAGCCTGAAAGAACTGGTGCTGAACGGCTGCACCGAACTGAAAAGCATTAACTGCCTGCCGCCGAACCTGAGCAGCCTGAGCATGGTGGGCTGCAGCAGCCTGGAAGTGATTAACTGCAGCATTCCGGAAAACGTGATTAACCTGAGCCTGTGCCATTGCAGCAGCCTGAAACATATTGAAGGCAGCTTTCCGGAAGCGCTGCGCAACAGCGTGTATCTGAACGGCTGCAACAGCCTGAACGAAAGCCAGTGCCAGTTTCTGGCGTATGATGTGAGCCAGGGCCGCGCGTGCCTGAGCAAAGCGGAACTGACCGCGGATCTGATTTGGCTGAGCGCGAACCGCACCGGCGAAGAAAGCGCGGAAGAACTGAACTATAGCGGCTGCGATCTGAGCGGCCTGAGCCTGGTGGGCCTGAACCTGAGCAGCGTGAACTTTAGCGGCGCGGTGCTGGATGATACCGATCTGCGCATGAGCGATCTGAGCCAGGCGGTGCTGGAAAACTGCAGCTTTAAAAACAGCATTCTGAACGAATGCAACTTTTGCTATGCGAACCTGAGCAACTGCATTATTCGCGCGCTGTTTGAAAACAGCAACTTTAGCAACAGCAACCTGAAAAACGCGAGCTTTAAAGGCAGCAGCTATATTCAGTATCCGCCGATTCTGAACGAAGCGGATCTGACCGGCGCGATTATTATTCCGGGCATGGTGCTGAGCGGCGCGATTCTGGGCGATGTGAAAGAACTGTTTAGCGAAAAAAGCAACACCATTAACCTGGGCGGCTGCTATATTGATCTGAGCGATATTCAGGAAAACATTCTGAGCGTGCTGGATAACTATACCAAAAGCAACAAAAGCATTCTGCTGACCATGAACACCAGCGATGATAAATATAACCATGATAAAGTGCGCGCGGCGGAAGAACTGATTAAAAAAATTAGCCTGGATGAACTGGCGGCGTTTCGCCCGTATGTGAAAATGAGCCTGGCGGATAGCTTTAGCATTCATCCGTATCTGAACAACGCGAACATTCAGCAGTGGCTGGAACCGATTTGCGATGATTTTTTTGATACCATTATGAGCTGGTTTAACAACAGCATTATGATGTATATGGAAAACGGCAGCCTGCTGCAGGCGGGCATGTATTTTGAACGCCATCCGGGCGCGATGGTGAGCTATAACAGCAGCTTTATTCAGATTGTGATGAACGGCAGCCGCCGCGATGGCATGCAGGAACGCTTTCGCGAACTGTATGAAGTGTATCTGAAAAACGAAAAAGTGTATCCGGTGACCCAGCAGAGCGATTTTGGCCTGTGCGATGGCAGCGGCAAACCGGATTGGGATGATGATAGCGATCTGGCGTATAACTGGGTGCTGCTGAGCAGCCAGGATGATGGCATGGCGATGATGTGCAGCCTGAGCCATATGGTGGATATGCTGAGCCCGAACACCAGCACCAACTGGATGAGCTTTTTTCTGTATAAAGATGGCGAAGTGCAGAACACCTTTGGCTATAGCCTGAGCAACCTGTTTAGCGAAAGCTTTCCGATTTTTAGCATTCCGTATCATAAAGCGTTTAGCCAGAACTTTGTGAGCGGCATTCTGGATATTCTGATTAGCGATAACGAACTGAAAGAACGCTTTATTGAAGCGCTGAACAGCAACAAAAGCGATTATAAAATGATTGCGGATGATCAGCAGCGCAAACTGGCGTGCGTGTGGAACCCGTTTCTGGATGGCTGGGAACTGAACGCGCAGCATGTGGATATGATTATGGGCAGCCATGTGCTGAAAGATATGCCGCTGCGCAAACAGGCGGAAATTCTGTTTTGCCTGGGCGGCGTGTTTTGCAAATATAGCAGCAGCGATATGTTTGGCACCGAATATGATAGCCCGGAAATTCTGCGCCGCTATGCGAACGGCCTGATTGAACAGGCGTATAAAACCGATCCGCAGGTGTTTGGCAGCGTGTATTATTATAACGATATTCTGGATCGCCTGCAGGGCCGCAACAACGTGTTTACCTGCACCGCGGTGCTGACCGATATGCTGACCGAACATGCGAAAGAAAGCTTTCCGGAAATTTTTAGCCTGTATTATCCGGTGGCGTGGCGCTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDENLYFQGGSNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLSSLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNESQCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSNFSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR*
Vector Type
Bacterial
Antibiotic
Amp
Comments
as described in http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019331. Codon optimised for E. coli
100042 R 1000 1001 1047 19007
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