USP6 | Ubiquitylation Resource

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Plasmid

pGEX6P-1-USP6 cat domain 529-1406

Parent Plasmid

pGEX6P1

Expressed

GST-USP6 cat domain

DU Number

DU20980

Genbank

9098

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCAAGGGAGCCACAGGTCTAAGCAACCTGGGAAACACATGCTTCAT
GAACTCAAGCATCCAGTGCGTTAGTAACACACAGCCACTGACACAGTATT
TTATCTCAGGGAGACATCTTTATGAACTCAACAGGACAAATCCCATTGGT
ATGAAGGGGCATATGGCTAAATGCTATGGTGATTTAGTGCAGGAACTCTG
GAGTGGAACTCAGAAGAGTGTTGCCCCATTAAAGCTTCGGCGGACCATAG
CAAAATATGCTCCCAAGTTTGATGGGTTTCAGCAACAAGACTCCCAAGAA
CTTCTGGCTTTTCTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCA
TGAAAAGCCATATGTGGAACTGAAGGACAGTGATGGCCGACCAGACTGGG
AAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGAAGAAATAGATCAATA
ATTGTGGATTTGTTCCATGGGCAGCTAAGATCTCAAGTCAAATGCAAGAC
ATGTGGGCATATAAGTGTCCGATTTGATCCTTTCAATTTTTTGTCTTTGC
CACTACCAATGGACAGTTACATGGACTTAGAAATAACAGTGATTAAGTTA
GATGGTACTACCCCTGTACGGTATGGACTAAGACTGAATATGGATGAAAA
GTACACAGGTTTAAAAAAACAGCTGAGGGATCTCTGTGGACTTAATTCAG
AACAAATCCTACTAGCAGAAGTACATGATTCCAACATAAAGAACTTTCCT
CAGGATAACCAAAAAGTACAACTCTCAGTGAGCGGATTTTTGTGTGCATT
TGAAATTCCTGTCCCTTCATCTCCAATTTCAGCTTCTAGTCCAACACAAA
TAGATTTCTCCTCTTCACCATCTACAAATGGAATGTTCACCCTAACTACC
AATGGGGACCTACCCAAACCAATATTCATCCCCAATGGAATGCCAAACAC
TGTTGTGCCATGTGGAACTGAGAAGAACTTCACAAATGGAATGGTTAATG
GTCACATGCCATCTCTTCCTGACAGCCCCTTTACAGGTTACATCATTGCA
GTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCACCTCAGGA
GAATCGCCCCAGCCTCTTTGGAATGCCATTGATTGTTCCATGCACTGTGC
ATACCCGGAAGAAAGACCTATATGATGCGGTTTGGATTCAAGTATCCTGG
TTAGCAAGACCACTCCCACCTCAGGAAGCTAGTATTCATGCCCAGGATCG
TGATAACTGTATGGGCTATCAATATCCATTCACTCTACGAGTTGTGCAGA
AAGATGGGAACTCCTGTGCTTGGTGCCCACAGTATAGATTTTGCAGAGGC
TGTAAAATTGATTGTGGGGAAGACAGAGCTTTCATTGGAAATGCCTATAT
TGCTGTGGATTGGCACCCCACAGCCCTTCACCTTCGCTATCAAACATCCC
AGGAAAGGGTTGTAGATAAGCATGAGAGTGTGGAGCAGAGTCGGCGAGCG
CAAGCCGAGCCCATCAACCTGGACAGCTGTCTCCGTGCTTTCACCAGTGA
GGAAGAGCTAGGGGAAAGTGAGATGTACTACTGTTCCAAGTGTAAGACCC
ACTGCTTAGCAACAAAGAAGCTGGATCTCTGGAGGCTTCCACCCTTCCTG
ATTATTCACCTTAAGCGATTTCAATTTGTAAATGATCAGTGGATAAAATC
ACAGAAAATTGTCAGATTTCTTCGGGAAAGTTTTGATCCGAGTGCTTTTT
TGGTACCACGAGACCCGGCCCTCTGCCAGCATAAACCACTCACACCCCAG
GGGGATGAGCTCTCCAAGCCCAGGATTCTGGCAAGAGAGGTGAAGAAAGT
GGATGCGCAGAGTTCGGCTGGAAAAGAGGACATGCTCCTAAGCAAAAGCC
CATCCTCACTCAGCGCTAACATCAGCAGCAGCCCAAAAGGTTCTCCTTCT
TCATCAAGAAAAAGTGGAACCAGCTGTCCCTCCAGCAAAAACAGCAGCCC
TAATAGCAGCCCACGGACTTTGGGGAGGAGCAAAGGGAGGCTCCGGCTGC
CCCAGATTGGCAGCAAAAATAAGCCGTCAAGTAGTAAGAAGAACTTGGAT
GCCAGCAAAGAGAATGGGGCTGGGCAGATCTGTGAGCTGGCTGACGCCTT
GAGCCGAGGGCATATGCGGGGGGGCAGCCAACCAGAGCTGGTCACTCCTC
AGGACCATGAGGTAGCTTTGGCCAATGGATTCCTTTATGAGCATGAAGCA
TGTGGCAATGGCTGTGGCGATGGCTACAGCAATGGTCAGCTTGGAAACCA
CAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGACACTCATATTAAGC
CTATTTATAATCTATATGCAATTTCATGCCATTCAGGAATTCTGAGTGGG
GGCCATTACATCACTTATGCCAAAAACCCAAACTGCAAGTGGTACTGTTA
TAATGACAGCAGCTGTGAGGAACTTCACCCTGATGAAATTGACACCGACT
CTGCCTACATTCTTTTCTATGAGCAGCAGGGGATAGACTACGCACAATTT
CTGCCAAAGATTGATGGCAAAAAGATGGCAGACACAAGCAGTACGGATGA
AGACTCTGAGTCTGATTACGAAAAGTACTCTATGTTACAGTAAGCGGCCG
C

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKGATGLSNLGNTCFMNSSI
QCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQ
KSVAPLKLRRTIAKYAPKFDGFQQQDSQELLAFLLDGLHEDLNRVHEKPY
VELKDSDGRPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHI
SVRFDPFNFLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGL
KKQLRDLCGLNSEQILLAEVHDSNIKNFPQDNQKVQLSVSGFLCAFEIPV
PSSPISASSPTQIDFSSSPSTNGMFTLTTNGDLPKPIFIPNGMPNTVVPC
GTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSPQENRPS
LFGMPLIVPCTVHTRKKDLYDAVWIQVSWLARPLPPQEASIHAQDRDNCM
GYQYPFTLRVVQKDGNSCAWCPQYRFCRGCKIDCGEDRAFIGNAYIAVDW
HPTALHLRYQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELG
ESEMYYCSKCKTHCLATKKLDLWRLPPFLIIHLKRFQFVNDQWIKSQKIV
RFLRESFDPSAFLVPRDPALCQHKPLTPQGDELSKPRILAREVKKVDAQS
SAGKEDMLLSKSPSSLSANISSSPKGSPSSSRKSGTSCPSSKNSSPNSSP
RTLGRSKGRLRLPQIGSKNKPSSSKKNLDASKENGAGQICELADALSRGH
MRGGSQPELVTPQDHEVALANGFLYEHEACGNGCGDGYSNGQLGNHSEED
STDDQREDTHIKPIYNLYAISCHSGILSGGHYITYAKNPNCKWYCYNDSS
CEELHPDEIDTDSAYILFYEQQGIDYAQFLPKIDGKKMADTSSTDEDSES
DYEKYSMLQ*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial expression construct to express USP6 cat domain with N-terminal GST tag (contains preScission protease site)

Estimated MW of expressed protein : 125297 Daltons

100042 R 1000 1001 1047 19007

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