USP9x | Ubiquitylation Resource

Synonyms

ubiquitin specific peptidase 9 X-linked

Plasmid

pGEX6P-1-USP9x 1553-1995 wt (corrected)

Parent Plasmid

pGEX6P1

Expressed

GST-USP9x 1553-1995 wt

DU Number

DU20628

Genbank

NP_001034680.2

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCCTGGAAGTTCTGTTCCAGGGCCCGAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTAAAACTGAAGATAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCTGCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLEVLFQGPKGFVGLKNAGA
TCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPR
DDVFGYPQQFEDKPALSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVP
RGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVL
GGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLE
GANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFND
YFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYR
LVGVLVHSGQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDE
EMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDE
LIRYISELAITTRPHQIIMPSAIERSVRKQN*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial expression construct for GST- USP9x-CAT DOMAIN WT

Estimated MW of expressed protein : 78786 Daltons

All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.

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