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TRIM27

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Plasmid
pMal-6His TRIM27
Parent Plasmid
pMAL6P
Expressed
MBP.6His-TRIM27
DU Number
DU20459
Tag
MBP
Species
Human
Sequence of Insert
GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCAC
CTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCG
GCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAG
ACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACAT
GCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGC
GCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAG
CACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTG
CGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGC
TCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGAC
CATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGA
ACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGA
TTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTAT
CGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCAT
CAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCC
TGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTG
CAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGA
ACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCC
AAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATG
CAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTC
AGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCT
CTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCT
GACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATG
CTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCA
AGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTA
ACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAA
AGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCC
CGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCC
TTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTAC
CTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGA
AAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTT
TCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGC
GGCCGC
Amino Acid Sequence
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFG
GYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYN
KDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYA
FKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFN
KGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINA
ASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIA
ATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNS
SSNNNNNNNNNNLGLEVLFQGPLGSMSYYHHHHHHDYDIPTTENLYFQGA
MGSMASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTA
ETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCE
KHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQL
DHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHE
YRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTREL
LQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEK
MQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDL
PDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGG
VTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEV
SFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDG
FSGHVGNHGHSMETSP*

Vector Type
Bacterial
Antibiotic
Amp
Comments
Construct for bacterial expression of N-terminally MBP.6HIS tagged TRIM27 (MBP-Precission-6HIS-TEV-TRIM27)

Estimated MW of expressed protein : 107561 Daltons
100042 R 1000 1001 1047 19007
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