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Plasmid
pET28-USP2b
Parent Plasmid
pET28a original
Expressed
6His-USP2b
DU Number
DU20875
Genbank
NM_171997.1*
Tag
6His
Sequence of Insert
View Sequence
GGATCCCCGAATTCCCGGGTCGACATGCGCACCTCGTACACCGTGACCCTGCCCGAGGACCCCCCCGCCGCCCCCTTTCCCGCCCTCGCCAAGGAGCTGCGGCCGCGCTCCCCTCTCTCCCCGTCCCTGCTGCTCTCCACCTTCGTGGGGCTCCTGCTCAACAAAGCCAAGAATTCTAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTGCAGTGCCTGAGCAACACTCGGGAGTTGAGAGATTACTGCCTCCAGAGGCTCTACATGCGGGACCTGCACCACGGCAGCAATGCACACACAGCCCTCGTGGAAGAGTTTGCAAAACTAATTCAGACCATATGGACTTCATCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCAGATCCAGAGATATGCACCGCGCTTTGTTGGCTATAATCAGCAGGATGCTCAGGAGTTCCTTCGCTTTCTTCTGGATGGGCTCCATAACGAGGTGAACCGAGTGACACTGAGACCTAAGTCCAACCCTGAGAACCTCGATCATCTTCCTGATGACGAGAAAGGCCGACAGATGTGGAGAAAATATCTAGAACGGGAAGACAGTAGGATCGGGGATCTCTTTGTTGGGCAGCTAAAGAGCTCGCTGACGTGTACAGATTGTGGTTACTGTTCTACGGTCTTCGACCCCTTCTGGGACCTCTCACTGCCCATTGCTAAGCGAGGTTATCCTGAGGTGACATTAATGGACTGCATGAGGCTCTTCACCAAAGAGGATGTGCTTGATGGAGATGAAAAGCCAACATGCTGTCGCTGCCGAGGCAGAAAACGGTGTATAAAGAAGTTCTCCATCCAGAGGTTCCCAAAGATCTTGGTGCTCCATCTGAAGCGGTTCTCAGAATCCAGGATCCGAACCAGCAAGCTCACAACATTTGTGAACTTCCCCCTAAGAGACCTGGACTTAAGAGAATTTGCCTCAGAAAACACCAACCATGCTGTTTACAACCTGTACGCTGTGTCCAATCACTCCGGAACCACCATGGGTGGCCACTATACAGCCTACTGTCGCAGTCCAGGGACAGGAGAATGGCACACTTTCAACGACTCCAGCGTCACTCCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTACCTGCTCTTCTACGAACTGGCCAGCCCGCCCTCCCGAATGTAGCGGCCGC
Amino Acid Sequence
View Sequence
HHHHHHSSGLVPRGSHMASMTGGQQMGRGSPNSRVDMRTSYTVTLPEDPP
AAPFPALAKELRPRSPLSPSLLLSTFVGLLLNKAKNSKSAQGLAGLRNLG
NTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQ
TIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEV
NRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSL
TCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEK
PTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPL
RDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTF
NDSSVTPMSSSQVRTSDAYLLFYELASPPSRM*
Vector Type
Bacterial
Antibiotic
Kan
100042 R 1000 1001 1047 19007
Download datasheet
Bacterial expression construct to express USP2b with N-terminal 6HIS tag (contains Thrombin cleavage site)
Estimated MW of expressed protein : 49091 Daltons