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Plasmid
pFBDual GST-Cullin2, 6His-Rbx1
Parent Plasmid
pFastbacDual 6P1
Expressed
GST-Cullin2
DU Number
DU21546
Genbank
NM_003591
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCatgtctttgaaaccaagagtagtagattttgatgaaacatggaacaaacttttgacgacaataaaagccgtggtcatgttggaatacgtcgaaagagcaacatggaatgaccgtttctcagatatctatgctttatgtgtggcctatcctgaaccccttggagaaagactttatacagaaactaagatttttttggaaaatcatgttcggcatttgcataagagagttttggagtcagaagaacaagtacttgttatgtatcataggtactgggaagaatacagcaagggtgcagactat
atggactgcttatataggtatctcaacacccagtttattaaaaagaataa
attaacagaagcggaccttcagtatggctatggtggtgtagatatgaatg
aaccacttatggaaataggagagctagcattggatatgtggaggaaattg
atggttgaaccacttcaggccatccttatccgaatgctgctccgagaaat
caaaaatgatcgtggtggagaagacccaaaccagaaagtaatccatgggg
ttattaactcctttgttcatgttgaacagtataagaaaaaattcccctta
aagttttatcaggaaatttttgagtctccctttctgactgaaacaggaga
gtattacaaacaagaagcttcaaatttattacaagaatcaaactgctcac
agtatatggaaaaggttctaggtagattaaaagatgaagaaattcgatgt
cgaaaatacctacatccaagttcatatactaaggtgattcatgaatgtca
acaacgaatggtagcagaccacttacagtttttacatgcagaatgtcata
atataattcgacaagagaaaaaaaatgacatggcaaatatgtacgtctta
ctccgtgctgtgtccactggtttacctcatatgattcaggagctgcaaaa
ccacatccatgatgagggccttcgagcaaccagcaaccttactcaggaaa
acatgccaacactatttgtggagtcagttttggaagtgcatggtaaattt
gttcagcttatcaacactgttttgaatggtgatcagcattttatgagtgc
gttggataaggcccttacgtcagttgtaaattacagagaacctaagtctg
tttgcaaagcacctgaactgcttgctaagtactgtgacaacttactgaag
aagtcagcgaaagggatgacagagaatgaagtggaagacaggctcacgag
cttcatcacagtgttcaaatacattgatgacaaggacgtctttcaaaagt
tctacgcaagaatgctggcaaaacgtttaattcatgggttatccatgtct
atggactctgaagaagccatgatcaacaaattaaagcaagcctgtggtta
tgagtttaccagcaagctacatcggatgtatacagatatgagtgtcagcg
ctgatctcaacaataagttcaacaattttatcaaaaaccaagacacagta
atagatttgggaattagttttcaaatatatgttctacaggctggtgcgtg
gcctcttactcaggctccttcatctacgtttgcaattccccaggaattag
aaaaaagtgtacagatgtttgaattattttatagccaacatttcagtgga
aggaaacttacatggttacattatctgtgtacaggtgaagttaaaatgaa
ctatttgggcaaaccatatgtagccatggttacaacataccaaatggcag
ttcttcttgcctttaacaacagtgaaactgtcagttataaagagcttcag
gacagcactcagatgaatgaaaaggaactgacaaaaacaatcaaatcatt
acttgatgtgaaaatgattaaccatgattcagaaaaggaagatattgatg
cagaatcttcgttttcattaaatatgaactttagcagtaaaagaacaaaa
tttaaaattactacatcaatgcagaaagacacaccacaagaaatggagca
gactagaagtgcagttgatgaggaccggaaaatgtatctccaagctgcta
tagttcgtatcatgaaagcacgaaaagtgcttcggcacaatgcccttatt
caagaggtgattagccagtcaagagctaggtttaatcccagtatcagcat
gattaagaagtgtattgaagttctgatagacaaacaatacatagaacgca
gccaggcgtcggcagatgaatacagctacgtcgcgtgagcggccgc
atggactgcttatataggtatctcaacacccagtttattaaaaagaataa
attaacagaagcggaccttcagtatggctatggtggtgtagatatgaatg
aaccacttatggaaataggagagctagcattggatatgtggaggaaattg
atggttgaaccacttcaggccatccttatccgaatgctgctccgagaaat
caaaaatgatcgtggtggagaagacccaaaccagaaagtaatccatgggg
ttattaactcctttgttcatgttgaacagtataagaaaaaattcccctta
aagttttatcaggaaatttttgagtctccctttctgactgaaacaggaga
gtattacaaacaagaagcttcaaatttattacaagaatcaaactgctcac
agtatatggaaaaggttctaggtagattaaaagatgaagaaattcgatgt
cgaaaatacctacatccaagttcatatactaaggtgattcatgaatgtca
acaacgaatggtagcagaccacttacagtttttacatgcagaatgtcata
atataattcgacaagagaaaaaaaatgacatggcaaatatgtacgtctta
ctccgtgctgtgtccactggtttacctcatatgattcaggagctgcaaaa
ccacatccatgatgagggccttcgagcaaccagcaaccttactcaggaaa
acatgccaacactatttgtggagtcagttttggaagtgcatggtaaattt
gttcagcttatcaacactgttttgaatggtgatcagcattttatgagtgc
gttggataaggcccttacgtcagttgtaaattacagagaacctaagtctg
tttgcaaagcacctgaactgcttgctaagtactgtgacaacttactgaag
aagtcagcgaaagggatgacagagaatgaagtggaagacaggctcacgag
cttcatcacagtgttcaaatacattgatgacaaggacgtctttcaaaagt
tctacgcaagaatgctggcaaaacgtttaattcatgggttatccatgtct
atggactctgaagaagccatgatcaacaaattaaagcaagcctgtggtta
tgagtttaccagcaagctacatcggatgtatacagatatgagtgtcagcg
ctgatctcaacaataagttcaacaattttatcaaaaaccaagacacagta
atagatttgggaattagttttcaaatatatgttctacaggctggtgcgtg
gcctcttactcaggctccttcatctacgtttgcaattccccaggaattag
aaaaaagtgtacagatgtttgaattattttatagccaacatttcagtgga
aggaaacttacatggttacattatctgtgtacaggtgaagttaaaatgaa
ctatttgggcaaaccatatgtagccatggttacaacataccaaatggcag
ttcttcttgcctttaacaacagtgaaactgtcagttataaagagcttcag
gacagcactcagatgaatgaaaaggaactgacaaaaacaatcaaatcatt
acttgatgtgaaaatgattaaccatgattcagaaaaggaagatattgatg
cagaatcttcgttttcattaaatatgaactttagcagtaaaagaacaaaa
tttaaaattactacatcaatgcagaaagacacaccacaagaaatggagca
gactagaagtgcagttgatgaggaccggaaaatgtatctccaagctgcta
tagttcgtatcatgaaagcacgaaaagtgcttcggcacaatgcccttatt
caagaggtgattagccagtcaagagctaggtttaatcccagtatcagcat
gattaagaagtgtattgaagttctgatagacaaacaatacatagaacgca
gccaggcgtcggcagatgaatacagctacgtcgcgtgagcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLKPRVVDFDETWNKLLT
TIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHV
RHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLT
EADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKN
DRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYY
KQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQR
MVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHI
HDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALD
KALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFI
TVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEF
TSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPL
TQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYL
GKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLD
VKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTR
SAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIK
KCIEVLIDKQYIERSQASADEYSYVA*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLKPRVVDFDETWNKLLT
TIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHV
RHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLT
EADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKN
DRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYY
KQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQR
MVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHI
HDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALD
KALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFI
TVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEF
TSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPL
TQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYL
GKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLD
VKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTR
SAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIK
KCIEVLIDKQYIERSQASADEYSYVA*
Vector Type
Baculovirus
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
(Cullin2 was subcloned BamHI-Not1 from SC20032 into the PPH driven cassette of SC20978 (pFBDual-GST6P-1 derived) thereby replacing the Cullin3 coding region). 6HIS-Rbx1 was previously subcloned Nhe1-Kpn1into the PP10 driven cassette.