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Plasmid
pETDuet-6His_TEV-Rbx1, GST_TEV-Cullin3 (codon optimised bacterial)
Parent Plasmid
pETDuet1
Expressed
GST-Cullin3
DU Number
DU23253
Tag
GST
Sequence of Insert
View Sequence
gtcgacatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatggatccGAAAACCTGTATTTTCAGATGAGCAATCTGAGCAAAGGCACCGGTTCCCGTAAAGACACCAAAATGCGTATCCGTGCGTTCCCGATGACGATGGACGAAAAATATGTGAACAGCATTTGGGATCTGCTGAAAAACGCGATTCAGGAAATCCAACGTAAAAACAATTCAGGTCTGTCGTTTGAAGAACTGTATCGCAATGCCTACACGATGGTGCTGCATAAACACGGCGAAAAACTGTATACCGGTCTGCGTGAAGTGGTTACGGAACATCTGATTAATAAAGTCCGCGAAGACGTGCTGAACAGCCTGAACAATAACTTTCTGCAGACCCTGAATCAAGCATGGAACGATCACCAGACGGCTATGGTGATGATTCGTGACATCCTGATGTATATGGATCGCGTTTACGTCCAGCAAAATAACGTGGAAAATGTTTATAACCTGGGTCTGATTATCTTCCGTGATCAGGTCGTGCGCTACGGCTGCATCCGTGACCATCTGCGCCAAACCCTGCTGGATATGATTGCCCGTGAACGCAAAGGCGAAGTTGTGGATCGTGGTGCGATCCGCAACGCCTGTCAGATGCTGATGATTCTGGGCCTGGAAGGTCGTTCAGTGTATGAAGAAGATTTCGAAGCGCCGTTTCTGGAAATGAGTGCCGAATTTTTCCAGATGGAATCCCAAAAATTTCTGGCAGAAAATAGTGCTTCCGTTTACATCAAAAAAGTCGAAGCACGTATTAACGAAGAAATCGAACGCGTCATGCACTGCCTGGATAAAAGCACCGAAGAACCGATTGTTAAAGTGGTTGAACGCGAACTGATCAGTAAACACATGAAAACCATCGTTGAAATGGAAAATTCCGGCCTGGTCCACATGCTGAAAAACGGCAAAACCGAAGACCTGGGTTGTATGTATAAACTGTTTAGTCGTGTGCCGAATGGTCTGAAAACCATGTGCGAATGTATGAGCAGCTACCTGCGCGAACAGGGCAAAGCACTGGTCTCCGAAGAAGGCGAAGGTAAAAACCCGGTGGATTATATCCAGGGTCTGCTGGACCTGAAATCACGTTTTGATCGCTTCCTGCTGGAATCGTTCAATAACGACCGTCTGTTTAAACAGACCATTGCGGGCGATTTTGAATATTTCCTGAATCTGAACTCACGCTCGCCGGAATACCTGAGTCTGTTTATCGATGACAAACTGAAAAAAGGCGTGAAAGGTCTGACCGAACAGGAAGTTGAAACGATTCTGGACAAAGCAATGGTTCTGTTTCGTTTCATGCAGGAAAAAGATGTCTTCGAACGCTATTACAAACAACATCTGGCTCGTCGCCTGCTGACCAATAAAAGCGTGTCTGATGACAGCGAGAAAAACATGATCTCTAAACTGAAAACCGAATGCGGCTGTCAGTTCACGAGCAAACTGGAAGGCATGTTTCGTGACATGAGTATTTCCAATACCACGATGGATGAATTTCGCCAGCACCTGCAAGCGACCGGTGTGTCACTGGGCGGTGTTGATCTGACGGTGCGTGTTCTGACCACGGGTTATTGGCCGACCCAGTCGGCAACGCCGAAATGCAACATCCCGCCGGCACCGCGTCATGCATTTGAAATTTTCCGTCGCTTTTATCTGGCCAAACACTCAGGCCGCCAGCTGACCCTGCAACATCACATGGGTTCGGCAGACCTGAATGCTACGTTCTACGGCCCGGTGAAAAAAGAAGATGGCAGCGAAGTCGGTGTGGGCGGTGCCCAGGTGACCGGTAGCAACACGCGTAAACATATCCTGCAGGTTTCTACCTTCCAAATGACGATTCTGATGCTGTTTAATAACCGCGAAAAATACACCTTCGAAGAAATCCAGCAAGAAACGGATATTCCGGAACGTGAACTGGTGCGCGCGCTGCAGAGCCTGGCATGTGGTAAACCGACCCAACGCGTTCTGACGAAAGAACCGAAATCTAAAGAAATCGAAAACGGCCATATCTTCACCGTGAACGACCAGTTTACGAGTAAACTGCACCGTGTCAAAATCCAGACCGTGGCGGCCAAACAGGGTGAATCCGATCCGGAACGTAAAGAAACGCGCCAGAAAGTGGATGACGATCGCAAACATGAAATTGAAGCAGCTATTGTTCGTATCATGAAAAGCCGTAAGAAAATGCAGCACAATGTTCTGGTCGCAGAAGTTACCCAGCAACTGAAAGCTCGTTTTCTGCCGTCTCCGGTCGTGATTAAAAAACGCATCGAAGGCCTGATTGAACGTGAATACCTGGCTCGCACCCCGGAAGATCGTAAAGTCTACACCTATGTCGCCTGAgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*
Vector Type
Bacterial
Antibiotic
Amp
Comments
both genes optimised for expression in E. coli, both genes preceded by Blunt TEV (ENLYFQ straight into Met)
100042 R 1000 1001 1047 19007
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