PEX10 | Ubiquitylation Resource

Plasmid

pEFIRES-P-eYFP C1-PEX10 a159c g165t c168g

Parent Plasmid

pEFIRES puro eYFPC1

Expressed

eYFP-PEX10 a159c g165t c168g

DU Number

DU20611

Genbank

NM_002617.3

Tag

YFP

Species

Human

Sequence of Insert

View Sequence

AGATCTATGGCCCCGGCCGCCGCCAGCCCCCCGGAGGTGATCCGCGCGGC
GCAGAAGGACGAGTACTACCGCGGTGGGCTGCGGAGCGCGGCGGGCGGCG
CCCTGCACAGCCTGGCGGGTGCGAGGAAGTGGCTGGAGTGGAGGAAGGAG
GTTGAGCTGCTCTCCGATGTTGCGTACTTTGGCCTCACCACACTTGCAGG
CTACCAGACCCTGGGGGAGGAGTACGTCAGCATCATCCAGGTGGACCCAT
CGCGGATACATGTGCCCTCCTCGCTGCGCCGTGGCGTGCTGGTGACACTG
CATGCCGTCCTGCCCTACCTGCTGGACAAGGCCCTGCTCCCCCTGGAGCA
GGAGCTGCAGGCTGACCCCGACAGTGGGCGACCCTTGCAGGGGAGCCTGG
GGCCAGGTGGGCGTGGCTGCTCAGGGGCGCGGCGCTGGATGCGTCACCAC
ACGGCCACCCTGACTGAGCAGCAGAGGAGGGCGCTGCTGCGGGCGGTCTT
CGTCCTCAGACAGGGCCTCGCCTGCCTCCAGCGGCTACATGTTGCCTGGT
TTTACATCCACGGTGTCTTCTACCACCTGGCCAAGAGGCTCACGGGGATC
ACGTACCTCCGTGTCCGCAGCCTGCCCGGAGAGGACCTGAGGGCCCGTGT
TAGCTACAGGCTGCTGGGGGTCATCTCACTGCTGCACCTGGTGCTGTCCA
TGGGGCTGCAGCTGTACGGTTTCAGGCAGCGGCAGCGAGCCAGGAAGGAG
TGGAGGCTGCACCGCGGCCTGTCTCACCGCAGGGCCTCCTTGGAGGAGAG
AGCCGTTTCCAGAAACCCCCTGTGCACCCTGTGCCTGGAGGAGCGCAGGC
ACCCAACAGCCACGCCCTGCGGCCACCTGTTCTGCTGGGAGTGCATCACC
GCGTGGTGCAGCAGCAAGGCGGAGTGTCCCCTCTGCCGGGAGAAGTTCCC
TCCCCAGAAGCTCATCTACCTTCGGCACTACCGCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMAPAAA
SPPEVIRAAQKDEYYRGGLRSAAGGALHSLAGARKWLEWRKEVELLSDVA
YFGLTTLAGYQTLGEEYVSIIQVDPSRIHVPSSLRRGVLVTLHAVLPYLL
DKALLPLEQELQADPDSGRPLQGSLGPGGRGCSGARRWMRHHTATLTEQQ
RRALLRAVFVLRQGLACLQRLHVAWFYIHGVFYHLAKRLTGITYLRVRSL
PGEDLRARVSYRLLGVISLLHLVLSMGLQLYGFRQRQRARKEWRLHRGLS
HRRASLEERAVSRNPLCTLCLEERRHPTATPCGHLFCWECITAWCSSKAE
CPLCREKFPPQKLIYLRHYR*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Mammalian expression vector for eYFP-PEX10 a159c g165t c168g. EF1a promoter, IRES and puromycin selection cassette

Estimated MW of expressed protein : 64544 Daltons

100042 R 1000 1001 1047 19007