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Plasmid
pET156P-1-USP9x 1553-1995 wt (with D1635G)
Parent Plasmid
pET15b 6His 3C
Expressed
6His-USP9x 1553-1995
DU Number
DU20657
Genbank
NP_001034680.2*
Tag
6His
Species
Human
Sequence of Insert
View Sequence
GGATCCCTGGAAGTTCTGTTCCAGGGCCCGAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTAAAACTGAAGGTAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCTGCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACTAG
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSLEVLFQGPKGFVGLKNAGATCYMNSVI
QQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQ
QFEDKPALSKTEGRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFR
LWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQK
ICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCE
KCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPREL
DMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHS
GQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFG
GEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISEL
AITTRPHQIIMPSAIERSVRKQN*
QQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQ
QFEDKPALSKTEGRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFR
LWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQK
ICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCE
KCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPREL
DMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHS
GQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFG
GEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISEL
AITTRPHQIIMPSAIERSVRKQN*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Bacterial expression construct to express USP9x 1553-1995 WT (with D1635G) with N-terminal 6HIS tag (contains preScission protease site)
Estimated MW of expressed protein : 54349 Daltons
All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.