USP49

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Plasmid
pET28a-HALO_TEV-USP49 C262A
Parent Plasmid
pET28a HALO TEV
Expressed
HALO-USP49 C262A
DU Number
DU23278
Tag
HALO
Sequence of Insert
ATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGACCACTCCATCCTGAACCCTCAGAAGTGGTGCTGCTTAGAGTGTGCCACCACCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGGCCGCTATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCTAGCCATGGAAGTCCGGGATCTCTACGTGTTCTGTTACCTGTGCAAGGACTACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGCTGCTAAGAAGCTCCCTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCGGACGCTGCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCGCTCCTCAGGGACAGCCGCAGATGCTCACGGCTCTGTGGTACCGGCGTCAGCGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCCGGGGCCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGAAGGAGGAGGCGCGGAGGCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAGGAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGGCTGCTCCTGCACACGCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCTCACGCAGAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATGGCCCCAGGCGTCACGGGCCTGCGCAACCTGGGCAACACCgcCTACATGAACTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGTTTCCTCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAACGGGAAGACTCAGCTTTCTGGCAAGCCAACCAACAGCTCGGCCACGGAGCTGTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCTGGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAGGAGCCGAGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTTCCGAGTCATGTGGTCCGGGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGCTGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCAACAGGACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACTCGAGTCTGAGGGCACCACACGCCGGATCCTCATCCCCTTCTCCCAGAGGAAGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCATATTTCATGGGCAGCTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCATTGAGCCCTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCATAGAAAAGGGGTTTGTCCCTTTGAATCAAACAGAGTGCTTGCTCACTGAGATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCTACGCTTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTGTTCTGAGTGAAGCTAGAAAGCAGTTAATGATCTACAGACTACCTCAGGTTCTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGCCGTAATCATCGAGAGAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTTACTGCTGCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTATGATCTCTCCGCAGTGGTCATGCATCACGGGAAAGGGTTTGGCTCAGGACACTACACAGCCTATTGCTACAACACAGAGGGAGGTGCGTGCGCTTTACTCTGTGGGGTGGGGGACACGGAAAGGGGTTGAGATCT
Amino Acid Sequence
MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTERG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
TGC to GCC (C262A) compared to ref shown
100042 R 1000 1001 1047 19007
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