optineurin

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Plasmid
pcDNA5-FRT/TO-DAC-A-C3 Optineurin
Parent Plasmid
pcDNA5 FRT/TO DAC 3C
Expressed
DAC-A-C3 Optineurin
DU Number
DU20573
Genbank
AF420371
Tag
DAC
Species
Human
Sequence of Insert
GGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAG
CCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACC
TGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTG
ACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCAT
GAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAG
AACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATG
GCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAA
AGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTC
CCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTG
AGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCA
GCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTA
GGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGT
CCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATATAG
GAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAA
CTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAG
AGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGA
AAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCA
CAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAA
GTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGA
GGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAG
AAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTG
AATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGT
GGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATG
AAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTCAA
GAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTC
AGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAAC
TGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAG
CAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAG
GGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGA
GAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTT
CTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGAT
GGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGG
CTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGG
AATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACAT
AGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence
MIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQA
MKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGIN
LQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADG
QYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSL
NVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLT
WGFRFFETVNPLEVLFQGPGSMSHQPLSCLTEKEDSPSESTGNGPPHLAH
PNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQ
KEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDS
RLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFV
EIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHE
ELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTE
GSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKR
LQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKT
EDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEK
LELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAER
AAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSD
QQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*


Vector Type
Mammalian
Antibiotic
Amp
Comments
Flp-In vector for mammalian expression of N-terminally DAC-A tagged optineurin

Estimated MW of expressed protein : 95410 Daltons
Parental Plasmid DU20573
100042 R 1000 1001 1047 19007
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