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Plasmid
pGEX6P1-Fan1 UBZ domain dimer (+ start and stop)
Parent Plasmid
pGEX6P1
Expressed
GST-Fan1 UBZ domain dimer
DU Number
DU20727
Genbank
NM_014967.3 GI:62198230
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GCCGGCAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAG
ATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCG
GTGGAGGTGGAGGTGGAGGCGCC
ATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCG
GTGGAGGTGGAGGTGGAGGCGCC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGKLACPVCSKMVPRYDL
NRHLDEMCANNDFGGGGGGGGKLACPVCSKMVPRYDLNRHLDEMCANNDF
*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGKLACPVCSKMVPRYDL
NRHLDEMCANNDFGGGGGGGGKLACPVCSKMVPRYDLNRHLDEMCANNDF
*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Also acts as Intermediate for further construction of UBZ domain concatamers
Estimated MW of expressed protein : 34290 Daltons
All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
Parental SC20707 (based on SC20103 pGEX6P-1 minus Sfo1 site)