ATG4B | Ubiquitylation Resource

Synonyms

APG4B, AUTL1, MGC1353

Plasmid

pBabe.puro-DU-GFP ATG4B

Parent Plasmid

pBabeD puro GFP

Expressed

GFP ATG4B

DU Number

DU36770

Genbank

NM_013325.4 GI:47132610*

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGA
GTTTGAAGATTTTCCTGAGACCTCAGAGCCCGTTTGGATACTGGGTAGAA
AATACAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCT
AGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGG
CCCCACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGA
TCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGG
ACACAAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATT
CATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGG
GAGTTGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAACACTGTC
GCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGC
GGTCCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGT
TGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGAT
TCCGACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAG
GCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGC
TCACGGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTGCTTCATG
ATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTA
CTTCATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCACACCA
CGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGC
TTCCACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCC
GTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATT
GGTGCCAGCAAGTCAAAAAGCTGTCTCTGCTCGGAGGTGCCCTGCCCATG
TTTGAGCTGGTGGAGCAGCAGCCTTCACATCTGGCCTGCCCCGACGTCCT
GAACCTGTCCCTAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTCG
ACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDAAT
LTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYR
KNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQP
DSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKL
AVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNG
FPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGV
IGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPP
CRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVEQQ
PSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL*

Vector Type

Mammalian

Antibiotic

Amp

Comments

*Sequence differences compared with NM_013325.4 CDS:
t1026c (silent)
t1061a (L354Q) – present in many published sequences for ATG4B
Retrovirus vector providing the viral package signal, transcription and processing elements and target gene. For expression in mammalian cells.

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