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Plasmid
pEGFP-C2-TAP Optineurin
Parent Plasmid
pEGFP TAP
Expressed
GFP-TAP-Optineurin
DU Number
DU4625
Genbank
AF420371
Tag
GFP
TAP
Species
Human
Sequence of Insert
View Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCCCAT
CAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCAC
AGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACCC
CGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCAG
CTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTGA
GGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTTG
AGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGAG
AATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAAG
GTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCGG
AGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGAG
AAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACTC
CAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGAG
AAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAGA
ACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAGA
TGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTCC
TGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGCA
CTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTAA
TCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAATG
ATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAAC
CTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAACT
CAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCCC
TTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGAG
CTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTATC
AGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTAA
CTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGCA
TTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACAG
GGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCTC
TGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAAG
CAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAGT
TTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGAGAGAGAAAATTCATG
AGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAAT
GATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTCG
TCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAAA
GAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCAT
TCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGAT
TCACGTGATGGATTGCATCATTTAAGCGGCCGC
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCCCAT
CAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCAC
AGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACCC
CGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCAG
CTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTGA
GGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTTG
AGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGAG
AATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAAG
GTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCGG
AGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGAG
AAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACTC
CAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGAG
AAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAGA
ACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAGA
TGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTCC
TGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGCA
CTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTAA
TCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAATG
ATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAAC
CTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAACT
CAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCCC
TTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGAG
CTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTATC
AGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTAA
CTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGCA
TTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACAG
GGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCTC
TGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAAG
CAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAGT
TTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGAGAGAGAAAATTCATG
AGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAAT
GATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTCG
TCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAAA
GAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCAT
TCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGAT
TCACGTGATGGATTGCATCATTTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMSH
QPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQ
LKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHE
NEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAE
KADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTR
TVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVA
LKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALN
LQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQE
LVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNA
LKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAK
QEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKEN
DAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIH
SCPKCGEVLPDIDTLQIHVMDCII*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMSH
QPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQ
LKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHE
NEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAE
KADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTR
TVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVA
LKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALN
LQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQE
LVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNA
LKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAK
QEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKEN
DAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIH
SCPKCGEVLPDIDTLQIHVMDCII*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Mammalian expression with GFP-TAP.
100042 R 1000 1001 1047 19007
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