USP8 | Ubiquitylation Resource

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Plasmid

pGEX6P-1-USP8

Parent Plasmid

pGEX6P1

Expressed

GST Precission USP8

DU Number

DU20323

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGCCTGCTGTGGCTTCAGTTCCTAAAGAACTCTACCTCAGTTC
TTCACTAAAAGACCTTAATAAGAAGACAGAAGTTAAACCAGAGAAAATAA
GCACTAAGAGTTATGTGCACAGTGCCCTGAAGATCTTTAAGACAGCAGAA
GAATGCAGATTAGATCGTGATGAGGAAAGGGCCTATGTACTATATATGAA
ATACGTGACTGTTTATAATCTTATCAAAAAAAGACCTGATTTCAAGCAAC
AGCAGGATTATTTCCATTCAATACTTGGACCTGGAAACATCAAAAAAGCT
GTCGAAGAAGCTGAAAGACTCTCTGAAAGCCTTAAATTAAGATATGAAGA
AGCTGAAGTCCGGAAAAAACTTGAGGAAAAAGACAGGCAGGAGGAAGCAC
AGCGGCTACAACAAAAAAGGCAGGAAACAGGAAGAGAGGATGGTGGCACA
TTGGCTAAAGGCTCTTTGGAGAATGTTTTGGATTCCAAAGACAAAACCCA
AAAGAGCAATGGTGAAAAGAATGAAAAATGTGAGACCAAAGAGAAAGGAGCAATCACAGCAAAGGAACTATACACAATGATGACGGATAAAAACATCAGCTTGATTATAATGGATGCTCGAAGAATGCAGGATTATCAGGATTCCTGTATTTTACATTCTCTCAGTGTTCCTGAAGAAGCCATCAGTCCAGGAGTCACTG
CTAGTTGGATTGAAGCACACCTGCCAGATGATTCTAAAGACACATGGAAG
AAGAGGGGGAATGTGGAGTATGTGGTACTTCTTGACTGGTTTAGTTCTGC
CAAAGATTTACAGATTGGAACAACTCTCCGGAGTCTGAAAGATGCACTTT
TCAAGTGGGAAAGTAAAACTGTCCTGCGCAATGAGCCTTTGGTTTTAGAG
GGAGGCTATGAAAACTGGCTCCTTTGTTATCCCCAGTATACAACAAATGC
TAAGGTCACTCCACCCCCACGACGCCAGAATGAAGAGGTGTCTATCTCAT
TGGATTTTACTTATCCCTCATTGGAAGAATCAATTCCTTCTAAACCTGCT
GCCCAGACGCCACCTGCATCTATAGAAGTAGATGAAAATATAGAATTGAT
AAGTGGTCAAAATGAGAGAATGGGACCACTGAATATATCAACTCCAGTTG
AACCAGTTGCTGCTTCTAAATCTGATGTTTCACCCATAATTCAGCCAGTG
CCTAGTATAAAGAATGTTCCACAGATTGATCGTACTAAAAAACCAGCAGT
CAAATTGCCTGAAGAGCATAGAATAAAATCTGAAAGTACAAACCATGAGC
AACAATCTCCTCAGAGTGGAAAAGTTATTCCTGATCGTTCCACCAAGCCA
GTAGTTTTTTCTCCAACTCTCATGTTAACAGATGAAGAAAAGGCTCGTAT
TCATGCAGAAACTGCTCTTCTAATGGAAAAAAACAAACAAGAAAAAGAAC
TTCGGGAAAGGCAGCAAGAGGAACAGAAAGAGAAACTGAGGAAGGAAGAACAAGAACAAAAAGCCAAAAAGAAACAAGAAGCTGAAGAAAATGAAATTACAGAGAAGCAACAAAAAGCAAAAGAAGAAATGGGGAAGAAAGAAAGTGAACAGGCCAAGAAAGAAGATAAAGAAACCTCAGCAAAGAGGGGCAAAGAAATAACAGGAGTAAAAAGACAAAGTAAAAGTGAACATGAAACTTCTGATGCCAAGAAATCTGTAGAAGATAGGGGGAAAAGGTGTCCAACCCCAGAAATACAGAAAAAGTCAACAGGAGATGTGCCCCATACATCTGTGACAGGGGATTCAGGTTCAGGCAAGCCATTTAAGATTAAAGGACAACCAGAAAGTGGAATTCTAAGGACAGGAACTTTTAGAGAGGATACAGACGATACCGAAAGAAATAAAGCTCAACGAGAACCTTTGACAAGAGCACGAAGTGAAGAAATGGGGAGGATCGTACCAGGACTGCCTTCAGGCTGGGCCAAGTTTCTTGACCCAATCACTGGAACCTTTCGTTATTATCATTCACCCACCAACACTGTTCATATGTACCCACCGGAAATGGCTCCTTCATCTGCACCTCCTTCCACCCCTCCAACTCATAAAGCC
AAGCCACAGATTCCTGCTGAGCGGGATAGGGAACCTTCCAAACTGAAGCG
CTCCTACTCCTCCCCAGATATAACCCAGGCTATTCAAGAGGAAGAGAAGA
GGAAGCCAACAGTAACTCCAACAGTTAATCGGGAAAACAAGCCAACATGT
TATCCTAAAGCTGAGATCTCAAGGCTTTCTGCTTCTCAGATTCGGAACCT
CAATCCTGTTTTTGGAGGTTCTGGACCAGCTCTTACTGGACTTCGTAACT
TAGGAAATACTTGTTATATGAACTCAATATTGCAGTGCCTATGTAACGCT
CCACATTTGGCTGATTATTTCAACCGAAACTGTTATCAGGATGATATTAA
CAGGTCAAATTTGTTGGGGCATAAAGGTGAAGTGGCAGAAGAATTTGGTA
TAATCATGAAAGCCCTGTGGACAGGACAGTATAGATATATCAGTCCAAAG
GACTTTAAAATCACCATTGGGAAGATCAATGACCAGTTTGCAGGATACAG
TCAGCAAGATTCACAAGAATTGCTTCTGTTCCTAATGGATGGTCTCCATG
AAGATCTAAATAAAGCTGATAATCGGAAGAGATATAAAGAAGAAAATAATGATCATCTCGATGACTTTAAAGCTGCAGAACATGCCTGGCAGAAACACAAGCAGCTCAATGAGTCTATTATTGTTGCACTTTTTCAGGGTCAATTCAAATCTACAGTACAGTGCCTCACATGTCACAAAAAGTCTAGGGCATTTGAGGCC
TTCATGTATTTGTCTCTACCACTAGCATCCACAAGTAAATGTACATTACA
GGATTGCCTTAGATTATTTTCCAAAGAAGAAAAACTCACAGATAACAACA
GATTTTACTGCAGTCATTGCAGAGCTCGACGGGATTCTCTAAAAAAGATA
GAAATCTGGAAGTTACCACCTGTGCTTTTAGTGCATCTGAAACGTTTTTC
CTACGATGGCAGGTGGAAACAAAAATTACAGACATCTGTGGACTTCCCGT
TAGAAAATCTTGACTTGTCACAGTATGTTATTGGTCCAAAGAACAATTTG
AAGAAATATAATTTGTTTTCTGTTTCAAATCACTACGGTGGGCTGGATGG
AGGCCACTACACAGCCTATTGTAAAAATGCAGCAAGACAACGGTGGTTTA
AGTTTGATGATCATGAAGTTTCTGATATCTCCGTTTCTTCTGTGAAATCT
TCAGCAGCTTATATCCTCTTTTATACTTCATTGGGACCACGAGTAACTGA
TGTAGCCACATAAGCGGCCGC

Amino Acid Sequence

View Sequence

LEVLFQGPLGSMPAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHS
ALKIFKTAEECRLDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSI
LGPGNIKKAVEEAERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQ
ETGREDGGTLAKGSLENVLDSKDKTQKSNGEKNEKCETKEKGAITAKELY
TMMTDKNISLIIMDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHL
PDDSKDTWKKRGNVEYVVLLDWFSSAKDLQIGTTLRSLKDALFKWESKTV
LRNEPLVLEGGYENWLLCYPQYTTNAKVTPPPRRQNEEVSISLDFTYPSL
EESIPSKPAAQTPPASIEVDENIELISGQNERMGPLNISTPVEPVAASKS
DVSPIIQPVPSIKNVPQIDRTKKPAVKLPEEHRIKSESTNHEQQSPQSGK
VIPDRSTKPVVFSPTLMLTDEEKARIHAETALLMEKNKQEKELRERQQEE
QKEKLRKEEQEQKAKKKQEAEENEITEKQQKAKEEMGKKESEQAKKEDKE
TSAKRGKEITGVKRQSKSEHETSDAKKSVEDRGKRCPTPEIQKKSTGDVP
HTSVTGDSGSGKPFKIKGQPESGILRTGTFREDTDDTERNKAQREPLTRA
RSEEMGRIVPGLPSGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAP
PSTPPTHKAKPQIPAERDREPSKLKRSYSSPDITQAIQEEEKRKPTVTPT
VNRENKPTCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMN
SILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWT
GQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLNKADN
RKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTC
HKKSRAFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCR
ARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQ
YVIGPKNNLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVS
DISVSSVKSSAAYILFYTSLGPRVTDVAT

Vector Type

Bacterial

Antibiotic

Amp

Comments

Estimated MW of expressed protein : 128587 Daltons

100042 R 1000 1001 1047 19007

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