USP22 | Ubiquitylation Resource

Plasmid

pFastbac GST USP22

Parent Plasmid

pFastbac GST

Expressed

GST USP22

DU Number

DU36273

Genbank

NM_015276.1 GI:150010638

Tag

GST

Species

Human

Sequence of Insert

View Sequence

ggatccatggtgtcccggccagagcccgagggcgaggccatggacgccga
gctggcggtagcgccgccgggctgctcgcacctgggcagcttcaaggtgg
acaactggaagcagaacctgcgggccatctaccagtgcttcgtgtggagc
ggcacggctgaggcccgcaagcgcaaggccaagtcctgtatctgccatgt
ctgtggcgtccacctcaacaggctgcattcctgcctctactgtgtcttct
tcggctgtttcacaaagaagcatattcacgagcatgcgaaggcgaagcgg
cacaacctggccattgatctgatgtacggaggcatctactgttttctgtg
ccaggactacatctatgacaaagacatggaaataatcgccaaggaggagc
agcgaaaagcttggaaaatgcaaggcgttggagagaagttttcaacttgg
gaaccaaccaaacgggagcttgaactgctgaagcacaacccgaaaaggag
aaagatcacctcgaactgcaccataggtctgcgtgggctgatcaaccttg
ggaacacatgcttcatgaactgcatcgtgcaggccctgacccacacgcca
cttctgcgggacttcttcctgtctgacaggcaccgctgtgagatgcagag
ccccagctcctgtctggtctgtgagatgtcctcactgtttcaggagtttt
actctggacaccggtcccctcacatcccgtataagttgctgcacctggtg
tggacccacgcgaggcacctagcaggctacgagcagcaggacgcccacga
gttcctcatcgcggccctggacgtgctccaccgacactgcaaaggtgatg
acaatgggaagaaggccaacaaccccaaccactgcaactgcatcatagac
cagatcttcacaggcgggttgcagtcagacgtcacctgccaagtctgcca
tggagtctccaccaccatcgaccccttctgggacatcagcttggatctcc
ccggctcttccaccccattctggcccctgagcccagggagcgagggcaac
gtggtaaacggggaaagccacgtgtcgggaaccaccacgctcacggactg
cctgcgacgattcaccagaccagagcacttgggcagcagcgccaagatca
agtgcagcggttgccatagctaccaggagtccacaaagcagctcactatg
aagaaactgcccatcgtagcctgttttcatctcaaacgatttgaacactc
agccaagctgcggcggaagatcaccacgtatgtgtccttccccctggagc
tggacatgacccctttcatggcctccagcaaagagagcaggatgaatgga
cagtaccagcagcccacggacagtctcaacaatgacaacaagtattccct
gtttgctgttgttaaccatcaagggaccttggagagtggccactacacca
gctttatccggcagcacaaagaccagtggttcaagtgtgacgatgccatc
atcaccaaggccagcatcaaggacgtcctggacagcgaagggtacttgct
gttctatcacaaacagttcctggaatacgagtaggtcgactcgagcggcc
gc

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVSRPEPEGEAMDAELAVA
PPGCSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCICHVCGVH
LNRLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYI
YDKDMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITS
NCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEMQSPSSC
LVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDAHEFLIA
ALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVST
TIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRF
TRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLR
RKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVV
NHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHK
QFLEYE*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

Baculovirus construct to express GST N terminal fusion

Estimated MW of expressed protein : 86782 Daltons

100042 R 1000 1001 1047 19007