USP28 | Ubiquitylation Resource

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Plasmid

pGEX6P-1-USP28 (Full Length)

Parent Plasmid

pGEX6P1

Expressed

GST 6P USP28

DU Number

DU20233

Genbank

AAH65928.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

ATGACTGCGGAGCTGCAGCAGGACGACGCGGCCGGCGCGGCAGACGGCCA
CGGCTCGAGCTGCCAAATGCTGTTAAATCAACTGAGAGAAATCACAGGCA
TTCAGGACCCTTCCTTTCTCCATGAAGCTCTGAAGGCCAGTAATGGTGAC
ATTACTCAGGCAGTCAGCCTTCTCACTGATGAGAGAGTTAAGGAGCCCAG
TCAAGACACTGTTGCTACAGAACCATCTGAAGTAGAGGGGAGTGCTGCCA
ACAAGGAAGTATTAGCAAAAGTTATAGACCTTACTCATGATAACAAAGAT
GATCTTCAGGCTGCCATTGCTTTGAGTCTACTGGAGTCTCCCAAAATTCA
AGCTGATGGAAGAGATCTTAACAGGATGCATGAAGCAACCTCTGCAGAAA
CTAAACGCTCAAAGAGAAAACGCTGTGAAGTCTGGGGAGAAAACCCCAAT
CCCAATGACTGGAGGAGAGTTGATGGTTGGCCAGTTGGGCTGAAAAATGT
TGGCAATACATGTTGGTTTAGTGCTGTTATTCAGTCTCTCTTTCAATTGC
CTGAATTTCGAAGACTTGTTCTCAGTTATAGTCTGCCACAAAATGTACTT
GAAAATTGTCGAAGTCATACAGAAAAGAGAAATATCATGTTTATGCAAGA
GCTTCAGTATTTGTTTGCTCTAATGATGGGATCAAATAGAAAATTTGTAG
ACCCGTCTGCAGCCCTGGATCTATTAAAGGGAGCATTCCGATCATCTGAG
GAACAGCAGCAAGATGTGAGTGAATTCACACACAAGCTCCTGGATTGGCT
AGAGGACGCATTCCAGCTAGCTGTTAATGTTAACAGTCCCAGGAACAAAT
CTGAAAATCCAATGGTGCAGCTGTTCTATGGTACTTTCCTGACTGAAGGG
GTTCGTGAAGGAAAACCCTTTTGTAACAATGAGACCTTCGGCCAGTATCC
TCTTCAGGTAAACGGTTATCGCAACTTAGACGAGTGTTTGGAAGGGGCCA
TGGTGGAGGGTGATGTTGAGCTTCTTCCCTCCGATCACTCGGTGAAGTAT
GGACAAGAGCGTTGGTTTACAAAGCTACCTCCAGTGTTGACCTTTGAACT
CTCAAGATTTGAGTTTAATCAGTCCCTTGGGCAGCCAGAGAAAATTCACA
ATAAGCTGGAATTTCCTCAGATTATTTATATGGACAGGTACATGTACAGG
AGCAAGGAGCTTATTCGAAATAAGAGAGAGTGTATTCGAAAGTTGAAGGA
GGAAATAAAAATTCTGCAGCAAAAATTGGAAAGGTATGTGAAATATGGCT
CAGGCCCAGCTCGGTTCCCGCTCCCGGACATGCTGAAATATGTTATTGAA
TTTGCTAGTACAAAACCTGCCTCAGAAAGCTGTCCACCTGAAAGTGACAC
ACATATGACATTACCACTTTCTTCAGTGCACTGCTCGGTTTCTGACCAGA
CATCCAAGGAAAGTACAAGTACAGAAAGCTCTTCTCAGGATGTTGAAAGT
ACCTTTTCTTCTCCTGAAGATTCTTTACCCAAGTCTAAACCACTGACATC
TTCTCGGTCTTCCATGGAAATGCCTTCACAGCCAGCTCCACGAACAGTCA
CAGATGAGGAGATAAATTTTGTTAAGACCTGTCTTCAGAGATGGAGGAGT
GAGATTGAACAAGATATACAAGATTTAAAGACTTGTATTGCAAGTACTAC
TCAGACTATTGAACAGATGTACTGCGATCCTCTCCTTCGTCAGGTGCCTT
ATCGCTTGCATGCAGTTCTTGTTCATGAAGGACAAGCAAATGCTGGACAC
TATTGGGCCTATATCTATAATCAACCCCGACAGAGCTGGCTCAAGTACAA
TGACATCTCTGTTACTGAATCTTCCTGGGAAGAAGTTGAAAGAGATTCCT
ATGGAGGCCTGAGAAATGTTAGTGCTTACTGTCTGATGTACATTAATGAC
AAACTACCCTACTTCAATGCAGAGGCAGCCCCAACTGAATCAGATCAAAT
GTCAGAAGTGGAAGCCCTATCTGTGGAACTCAAGCATTACATTCAGGAGG
ATAACTGGCGGTTTGAGCAGGAAGTAGAGGAGTGGGAAGAAGAGCAGTCT
TGCAAAATCCCTCAAATGGAGTCCTCCACCAACTCCTCATCACAGGACTA
CTCTACATCACAAGAGCCTTCAGTAGCCTCTTCTCATGGGGTTCGCTGCT
TGTCATCTGAGCATGCTGTGATTGTAAAGGAGCAAACTGCCCAGGCTATT
GCAAACACAGCCCGTGCCTATGAGAAGAGCGGTGTAGAAGCGGCACTGAG
TGAGGTGATGCTGAGCCCTGCCATGCAAGGGGTCATCCTGGCCATAGCTA
AAGCCCGTCAGACCTTTGACCGAGATGGGTCTGAAGCAGGGCTGATTAAG
GCATTCCATGAAGAATACTCCAGGCTCTATCAGCTTGCCAAAGAGACCCC
CACCTCTCACAGTGATCCTCGACTTCAGCATGTCCTTGTCTACTTTTTCC
AAAATGAAGCACCCAAAAGGGTAGTAGAACGAACCCTTCTGGAACAGTTT
GCAGATAAAAATCTTAGCTATGATGAAAGATCAATCAGCATTATGAAGGT
GGCTCAAGCGAAACTGAAGGAAATTGGTCCAGATGACATGAATATGGAAG
AGTACAAGAAGTGGCATGAAGATTATAGTTTGTTCCGAAAAGTGTCTGTG
TATCTCCTAACAGGCCTAGAACTCTATCAAAAAGGAAAGTACCAAGAGGC
ACTTTCCTACCTGGTATATGCCTACCAGAGCAATGCTGCCCTGCTGATGA
AGGGGCCCCGCCGGGGGGTCAAAGAATCCGTGATTGCTTTATACCGAAGA
AAATGCCTTCTGGAGCTGAATGCCAAAGCAGCTTCTCTTTTTGAAACAAA
TGATGATCACTCCGTAACTGAGGGCATTAATGTGATGAATGAACTGATCA
TCCCCTGCATTCACCTTATCATTAATAATGACATTTCCAAGGATGATCTG
GATGCCATTGAGGTCATGAGAAACCATTGGTGCTCTTACCTTGGGCAAGA
TATTGCAGAAAATCTGCAGCTGTGCCTAGGGGAGTTTCTACCCAGACTTC
TAGATCCTTCTGCAGAAATCATCGTCTTGAAAGAGCCTCCAACTATTCGA
CCCAATTCTCCCTATGACCTATGTAGCCGATTTGCAGCTGTCATGGAGTC
AATTCAGGGAGTTTCAACTGTGACAGTGAAATAA

Amino Acid Sequence

View Sequence

MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGD
ITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKD
DLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPN
PNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVL
ENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSE
EQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEG
VREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKY
GQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYR
SKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIE
FASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVES
TFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRS
EIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGH
YWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYIND
KLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQS
CKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAI
ANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIK
AFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQF
ADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSV
YLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRR
KCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDL
DAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIR
PNSPYDLCSRFAAVMESIQGVSTVTVK*

Vector Type

Bacterial

Comments

Sub-cloning of 3’ fragment into DU12808 (5’Fragment) using internal Nde1 site in USP28 sequence.
Silent mutation g2205a.

Estimated MW of expressed protein : 122456.92 Daltons

100042 R 1000 1001 1047 19007

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