USP30 | Ubiquitylation Resource

Synonyms

FLJ40511, MGC10702

Plasmid

pGEX6P-USP30 57-end (lacks transmembrane domain)

Parent Plasmid

pGEX6P3

Expressed

GST-USP30 57-end

DU Number

DU36294

Genbank

NM_032663.3 GI:164607166

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCTGGCCTTGT
TAATTTAGGGAACACCTGCTTCATGAACTCCCTGCTACAAGGCCTGTCTG
CCTGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCC
AGGGATCAGAAGGAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTT
GCACCTTCTGAAAGCCTTGTCCTGCCAAGAAGTTACTGATGATGAGGTCT
TAGATGCAAGCTGCTTGTTGGATGTCTTAAGAATGTACAGATGGCAGATC
TCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTCATTACCTC
GTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTG
ATGTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAAATTACC
TGCCGCACAAGAGGGTCACCTCACCCTACATCCAATCACTGGAAGTCTCA
ACATCCTTTTCATGGAAGACTCACTAGTAATATGGTCTGCAAACACTGTG
AACACCAGAGTCCTGTTCGATTTGATACCTTTGATAGCCTTTCACTAAGT
ATTCCAGCCGCCACATGGGGTCACCCATTGACCCTGGACCACTGCCTTCA
CCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTA
CAAAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAG
AGGACCACTTTTGTTAAACAGTTAAAACTAGGGAAGCTCCCTCAGTGTCT
CTGCATCCACCTACAGCGGCTGAGCTGGTCCAGCCACGGCACGCCTCTGA
AGCGGCATGAGCACGTGCAGTTCAATGAGTTCCTGATGATGGACATTTAC
AAGTACCACCTCCTTGGACATAAACCTAGTCAACACAACCCTAAACTGAA
CAAGAACCCAGGGCCTACACTGGAGCTGCAGGATGGGCCGGGAGCCCCCA
CACCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAAT
GGCGCCTGCTCCCCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTT
CCCTCTCCCAGTTGTTCCCGACTACAGCTCCTCCACATACCTCTTCCGGC
TGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTC
ACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAA
TCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGG
AGGTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTTCC
AGGATGCAGCACCAGAGCCAGGAGTGCAAGTCTGAAGAATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTERKKRRKGLVPGLVNLGN
TCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLK
ALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLED
ERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFH
GRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFIS
SESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHL
QRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPG
PTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPV
VPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQWLW
VSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Bacterial construct to express GST N terminal fusion

Estimated MW of expressed protein : 79407 Daltons

100042 R 1000 1001 1047 19007