USP52 | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-FLAG USP52

Parent Plasmid

pcDNA5 FRT/TO FLAG2

Expressed

FLAG-USP52

DU Number

DU14312

Genbank

NP_001120932.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCCATGAACTTTGAGGGTCTGGACCCTGGACTGGCAGAATATGC
CCCAGCCATGCATTCTGCCCTGGACCCTGTCTTGGATGCCCACCTGAACC
CAAGTCTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCCTTGGAG
GCTCTTCCCGTCCAGGAATCAGTGCACATAATGGAAGGTGTCTACTCTGA
ATTGCACAGCGTGGTGGCTGAAGTGGGTGTACCTGTTTCCGTCTCCCACT
TTGACTTGCACGAGGAGATGCTGTGGGTGGGGAGCCACGGGGGCCATGCC
ACTTCATTTTTTGGCCCAGCCTTGGAGCGCTACTCATCCTTTCAAGTCAA
TGGCAGTGATGATATTCGGCAGATCCAGAGCCTGGAGAATGGTATCCTTT
TTCTCACCAAGAACAACCTCAAGTATATGGCCCGTGGGGGCCTCATTATA
TTTGATTACCTGCTGGATGAGAATGAGGATATGCACAGTCTCCTACTGAC
TGACAGCAGCACTCTACTCGTTGGTGGGCTGCAGAATCACATACTAGAGA
TTGATCTTAACACTGTCCAGGAGACTCAGAAGTATGCAGTAGAGACGCCT
GGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTCTGCGGCCACAC
GTCTGGCAAGGTTTCCCTGAGAGACCTCCGTACTTTTAAGGTGGAACATG
AGTTTGATGCCTTCTCAGGAAGTCTGTCAGACTTTGATGTGCATGGCAAC
CTGCTAGCTGCCTGTGGCTTCTCCAGCCGCCTCACTGGCCTGGCCTGCGA
CCGTTTCCTCAAGGTGTATGATTTGCGCATGATGCGTGCCATCACACCAC
TTCAAGTACATGTGGATCCTGCCTTCTTGCGCTTCATTCCTACATATACT
TCTCGTCTTGCTATCATCTCTCAGTCAGGGCAGTGCCAATTCTGTGAACC
CACAGGCCTGGCCAACCCAGCCGATATCTTTCATGTGAATCCTGTGGGGC
CTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCTCTGGCCTTT
GGGGATTCTGAGGGCTGTGTGCACCTCTGGACTGATTCCCCGGAGCCTTC
CTTCAACCCCTACTCCCGTGAGACTGAGTTTGCTTTGCCGTGTCTCGTGG
ACTCACTGCCTCCTCTGGACTGGAGCCAGGACCTGCTGCCTCTTTCCCTC
ATCCCTGTCCCACTCACCACTGACACACTTCTCTCTGATTGGCCTGCTGC
CAACTCTGCTCCAGCTCCCAGGCGAGCACCACCCGTGGATGCAGAGATTC
TGCGCACCATGAAGAAGGTGGGCTTCATTGGCTATGCGCCCAATCCCCGC
ACCAGGCTGCGCAATCAGATACCCTACAGACTCAAGGAGTCAGACAGTGA
ATTTGACAGCTTCAGCCAGGTCACTGAGTCACCAGTAGGACGAGAAGAGG
AACCACATCTCCACATGGTTTCTAAGAAATACCGCAAGGTGACCATCAAA
TATTCCAAGCTAGGGCTGGAGGACTTTGACTTCAAACACTACAATAAGAC
CTTGTTTGCTGGATTAGAGCCCCACATTCCCAACGCCTACTGTAACTGCA
TGATCCAGGTGCTCTATTTCCTGGAGCCTGTACGCTGTCTAATTCAAAAC
CACCTTTGCCAGAAGGAGTTCTGTCTGGCATGTGAGCTGGGCTTCCTGTT
TCACATGTTGGACCTCTCTCGTGGTGACCCTTGCCAGGGCAATAATTTTC
TTCGGGCATTCCGTACTATTCCTGAGGCCTCAGCCCTCGGTCTAATCCTG
GCTGACTCAGATGAGGCCTCAGGCAAGGGCAATCTGGCCAGGCTCATTCA
GAGGTGGAATCGCTTCATTCTCACTCAACTGCATCAAGATATGCAGGAGC
TGGAAATACCACAGGCTTATCGAGGTGCTGGAGGCAGCAGCTTTTGCTCA
TCGGGGGACTCTGTTATTGGGCAGCTCTTCAGCTGTGAGATGGAGAACTG
CAGCCTCTGCCGCTGTGGCAGTGAGACCGTGCGAGCCTCATCCACTCTGC
TTTTCACACTCTCCTACCCTGATGGTAGCAAAAGTGATAAAACTGGGAAG
AACTATGACTTTGCTCAGGTGCTGAAGCGAAGCATCTGCCTGGACCAGAA
TACACAGGCCTGGTGTGACACCTGTGAAAAGTACCAGCCCACGATTCAGA
CCCGCAACATCCGCCATCTGCCAGATATTCTTGTCATCAATTGTGAGGTG
AACAGCTCAAAAGAGGCTGATTTCTGGAGAATGCAGGCTGAGGTTGCCTT
CAAGATGGCAGTAAAGAAACACGGTGGGGAAATCTCCAAGAACAAGGAAT
TTGCTTTGGCTGATTGGAAGGAACTAGGGAGTCCAGAGGGTGTGCTGGTG
TGTCCCTCCATTGAGGAGTTGAAGAACGTCTGGCTTCCTTTCTCCATTCG
CATGAAGATGACCAAAAACAAAGGGCTGGATGTTTGCAATTGGACTGATG
GGGATGAGATGCAGTGGGGCCCAGCCAGGGCAGAGGAGGAGCATGGTGTC
TATGTGTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGCAC
AGGGGGCAGCCTGGTGGCTCACATCAAAGTTGGAGAGACCTACCACCAGC
GCAAGGAGGGCGTTACTCACCAGCAGTGGTATCTGTTCAATGACTTTCTT
ATTGAACCTATTGATAAGCATGAAGCTGTGCAGTTTGACATGAATTGGAA
AGTACCTGCAATCCTTTATTATGTCAAACGGAATCTCAATTCCAGATACA
ACCTGAACATCAAGAACCCTATTGAGGCAAGTGTCTTGCTGGCTGAAGCC
TCGCTGGCACGGAAGCAGCGGAAAACACATACTACCTTTATTCCACTGAT
GCTGAATGAGATGCCACAGATTGGGGACCTGGTGGGTCTGGATGCTGAGT
TTGTCACCCTTAATGAGGAGGAAGCAGAGTTACGCAGTGATGGTACCAAG
TCTACCATTAAACCAAGCCAGATGTCAGTAGCCAGGATTACCTGTGTTCG
GGGCCAGGGACCCAATGAGGGTATCCCCTTCATTGATGACTACATCTCTA
CCCAGGAGCAGGTGGTGGATTACTTGACTCAATACTCGGGTATAAAGCCT
GGTGACCTCGATGCCAAAATTTCCTCCAAGCACCTAACAACTCTCAAGTC
TACCTACTTAAAGCTTCGTTTTCTCATTGACATTGGAGTCAAGTTTGTGG
GTCATGGCCTGCAGAAGGACTTCCGGGTCATCAACCTGATGGTGCCCAAG
GACCAAGTCCTTGACACTGTCTACCTGTTCCATATGCCCCGAAAACGAAT
GATTTCCCTGCGATTCCTTGCTTGGTACTTTCTGGACCTGAAGATTCAAG
GGGAAACCCATGACAGTATTGAGGATGCCCGCACAGCCCTTCAGCTGTAC
CGAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTGAGTCTTTCCA
CAAGGTGCTCAAGGGTCTTTATGAGAAGGGCAGAAAGATGGACTGGAAGG
TGCCTGAGCCTGAGGGCCAAACAAGTCCCAAGAATGCAGCTGTCTTCTCC
TCAGTGCTGGCGCTCTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGIPGIPGSTRAAAMNFEGLDPGLAEYAPAMHSALDPVLDAH
LNPSLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVAEVGVPVSV
SHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENG
ILFLTKNNLKYMARGGLIIFDYLLDENEDMHSLLLTDSSTLLVGGLQNHI
LEIDLNTVQETQKYAVETPGVTIMRQTNRFFFCGHTSGKVSLRDLRTFKV
EHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAI
TPLQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNP
VGPLLMTFDVSASKQALAFGDSEGCVHLWTDSPEPSFNPYSRETEFALPC
LVDSLPPLDWSQDLLPLSLIPVPLTTDTLLSDWPAANSAPAPRRAPPVDA
EILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDSEFDSFSQVTESPVGR
EEEPHLHMVSKKYRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYC
NCMIQVLYFLEPVRCLIQNHLCQKEFCLACELGFLFHMLDLSRGDPCQGN
NFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDM
QELEIPQAYRGAGGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASS
TLLFTLSYPDGSKSDKTGKNYDFAQVLKRSICLDQNTQAWCDTCEKYQPT
IQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKKHGGEISKN
KEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNW
TDGDEMQWGPARAEEEHGVYVYDLMATVVHILDSRTGGSLVAHIKVGETY
HQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAILYYVKRNLNS
RYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLD
AEFVTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDY
ISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDIGVK
FVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYFLDLK
IQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMD
WKVPEPEGQTSPKNAAVFSSVLAL*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 137639 Daltons

100042 R 1000 1001 1047 19007

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