USP27X | Ubiquitylation Resource

Synonyms

USP22L, USP27

Plasmid

pEBG6P-USP27X

Parent Plasmid

pEBG6P

Expressed

GST-USP27X

DU Number

DU36342

Genbank

NM_001145073.1 GI:223005905

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGTGTAAGGACTATGTATATGACAAAGACATTGAGCAAATTGC
CAAAGAAGAGCAAGGAGAAGCTTTGAAATTACAAGCCTCCACCTCAACAG
AGGTTTCTCACCAGCAGTGTTCAGTGCCAGGCCTTGGTGAGAAATTCCCA
ACCTGGGAAACAACCAAACCAGAATTAGAACTGCTGGGGCACAACCCGAG
GAGAAGAAGAATCACCTCCAGCTTTACGATCGGTTTAAGAGGACTCATCA
ATCTTGGCAACACGTGCTTTATGAACTGCATTGTCCAGGCCCTCACCCAC
ACGCCGATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGATGTGAGAT
GCCGAGTCCCGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTGTTTCGGG
AGTTGTATTCTGGAAACCCGTCTCCTCATGTGCCCTATAAGTTACTGCAC
CTGGTGTGGATACATGCCCGCCATTTAGCAGGGTACAGGCAACAGGATGC
CCACGAGTTCCTCATTGCAGCGTTAGATGTCCTGCACAGGCACTGCAAAG
GTGATGATGTCGGGAAGGCGGCCAACAATCCCAACCACTGTAACTGCATC
ATAGACCAAATCTTCACAGGTGGCCTGCAGTCTGATGTCACCTGTCAAGC
CTGCCATGGCGTCTCCACCACGATAGACCCATGCTGGGACATTAGTTTGG
ACTTGCCTGGCTCTTGCACCTCCTTCTGGCCCATGAGCCCAGGGAGGGAG
AGCAGTGTGAACGGGGAAAGCCACATACCAGGAATCACCACCCTCACGGA
CTGCTTGCGGAGGTTTACGAGGCCAGAGCACTTAGGAAGCAGTGCCAAAA
TCAAATGTGGTAGTTGCCAAAGCTACCAGGAATCTACCAAACAGCTCACA
ATGAATAAATTACCTGTCGTTGCCTGTTTTCATTTCAAACGGTTTGAACA
TTCAGCGAAACAGAGGCGCAAGATCACTACATACATTTCCTTTCCTCTGG
AGCTGGATATGACGCCGTTTATGGCCTCAAGTAAAGAGAGCAGAATGAAT
GGACAATTGCAGCTGCCAACCAATAGTGGAAACAACGAAAATAAGTATTC
CTTGTTTGCTGTGGTTAATCACCAAGGAACCTTGGAGAGTGGCCACTATA
CCAGCTTCATCCGGCACCACAAGGACCAGTGGTTCAAGTGTGATGATGCC
GTCATCACTAAGGCCAGTATTAAGGACGTACTGGACAGTGAAGGGTATTT
ACTGTTCTATCACAAACAGGTGCTAGAACATGAGTCAGAAAAAGTGAAAG
AAATGAACACACAAGCCTACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMCKDYVYDKDIEQ
IAKEEQGEALKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHN
PRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRC
EMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQ
DAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTC
QACHGVSTTIDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTL
TDCLRRFTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRF
EHSAKQRRKITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENK
YSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEG
YLLFYHKQVLEHESEKVKEMNTQAY*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Mammalian expression plasmid to express USP27X with N-terminal GST (contains preScission protease site)

Estimated MW of expressed protein : 77046 Daltons

100042 R 1000 1001 1047 19007