USP30 | Ubiquitylation Resource

Plasmid

pFastbac GST USP30 (includes transmembrane domain)

Parent Plasmid

pFastbac GST

Expressed

GST-USP30

DU Number

DU36244

Genbank

NM_032663.3 GI:164607166

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGCTGAGCTCCCGGGCCGAGGCGGCGATGACCGCGGCCGACAG
GGCCATCCAGCGCTTCCTGCGGACCGGGGCGGCCGTCAGATATAAAGTCA
TGAAGAACTGGGGAGTTATAGGTGGAATTGCTGCTGCTCTTGCAGCAGGA
ATATATGTTATTTGGGGTCCCATTACAGAAAGAAAGAAGCGTAGAAAAGG
GCTTGTGCCTGGCCTTGTTAATTTAGGGAACACCTGCTTCATGAACTCCC
TGCTACAAGGCCTGTCTGCCTGTCCTGCTTTCATCAGGTGGCTGGAAGAG
TTCACCTCCCAGTACTCCAGGGATCAGAAGGAGCCCCCCTCACACCAGTA
TTTATCCTTAACACTCTTGCACCTTCTGAAAGCCTTGTCCTGCCAAGAAG
TTACTGATGATGAGGTCTTAGATGCAAGCTGCTTGTTGGATGTCTTAAGA
ATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATT
ATTCCATGTCATTACCTCGTCATTGGAAGATGAGCGAGACCGCCAGCCTC
GGGTCACACATTTGTTTGATGTGCATTCCCTGGAGCAGCAGTCAGAAATA
ACTCCCAAACAAATTACCTGCCGCACAAGAGGGTCACCTCACCCTACATC
CAATCACTGGAAGTCTCAACATCCTTTTCATGGAAGACTCACTAGTAATA
TGGTCTGCAAACACTGTGAACACCAGAGTCCTGTTCGATTTGATACCTTT
GATAGCCTTTCACTAAGTATTCCAGCCGCCACATGGGGTCACCCATTGAC
CCTGGACCACTGCCTTCACCACTTCATCTCATCAGAATCAGTGCGGGATG
TTGTGTGTGACAACTGTACAAAGATTGAAGCCAAGGGAACGTTGAACGGG
GAAAAGGTGGAACACCAGAGGACCACTTTTGTTAAACAGTTAAAACTAGG
GAAGCTCCCTCAGTGTCTCTGCATCCACCTACAGCGGCTGAGCTGGTCCA
GCCACGGCACGCCTCTGAAGCGGCATGAGCACGTGCAGTTCAATGAGTTC
CTGATGATGGACATTTACAAGTACCACCTCCTTGGACATAAACCTAGTCA
ACACAACCCTAAACTGAACAAGAACCCAGGGCCTACACTGGAGCTGCAGG
ATGGGCCGGGAGCCCCCACACCAGTTCTGAATCAGCCAGGGGCCCCCAAA
ACACAGATTTTTATGAATGGCGCCTGCTCCCCATCTTTATTGCCAACGCT
GTCAGCGCCGATGCCCTTCCCTCTCCCAGTTGTTCCCGACTACAGCTCCT
CCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATG
CACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAA
CCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCC
GCAAGGCCAGCCTGCAGGAGGTCCTGTCCTCCAGCGCCTACCTGCTGTTC
TACGAGCGCGTCCTTTCCAGGATGCAGCACCAGAGCCAGGAGTGCAAGTC
TGAAGAATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLSSRAEAAMTAADRAIQR
FLRTGAAVRYKVMKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPG
LVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLT
LLHLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVI
TSSLEDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWK
SQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHC
LHHFISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQ
CLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPK
LNKNPGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPM
PFPLPVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLST
SNQWLWVSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE*

Vector Type

Baculovirus

Antibiotic

Amp

Comments

Baculovirus construct to express GST N terminal fusion

Estimated MW of expressed protein : 85324 Daltons

100042 R 1000 1001 1047 19007