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USP49

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Plasmid
pFastbac GST USP49
Parent Plasmid
pFastbac GST
Expressed
GST-USP49
DU Number
DU36247
Genbank
NM_018561.3 GI:34147686
Tag
GST
Species
Human
Sequence of Insert
AGATCTATGGATAGATGCAAACATGTAGGGCGGTTACGGCTCGCCCAGGA
CCACTCCATCCTGAACCCTCAGAAGTGGTGCTGCTTAGAGTGTGCCACCA
CCGAGTCCGTGTGGGCCTGCCTCAAGTGCTCCCACGTGGCCTGCGGCCGC
TATATTGAGGACCACGCCCTGAAACACTTTGAGGAGACGGGACACCCGCT
AGCCATGGAAGTCCGGGATCTCTACGTGTTCTGTTACCTGTGCAAGGACT
ACGTGCTCAATGATAACCCAGAGGGGGACCTGAAGCTGCTAAGAAGCTCC
CTCCTGGCGGTCCGGGGCCAGAAACAGGACACGCCGGTGAGACGTGGGCG
GACGCTGCGGTCCATGGCTTCGGGTGAGGACGTGGTCCTGCCGCAGCGCG
CTCCTCAGGGACAGCCGCAGATGCTCACGGCTCTGTGGTACCGGCGTCAG
CGCCTGCTGGCCAGGACGCTGCGGCTGTGGTTCGAGAAGAGCTCCCGGGG
CCAGGCGAAGCTGGAGCAGCGGCGGCAGGAGGAGGCCCTGGAGCGCAAGA
AGGAGGAGGCGCGGAGGCGGCGGCGCGAGGTGAAACGGCGGCTGCTGGAG
GAGCTGGCCAGCACCCCTCCGCGCAAGAGTGCACGGCTGCTCCTGCACAC
GCCCCGCGACGCGGGCCCGGCTGCCTCGCGCCCCGCCGCCCTCCCTACCT
CACGCAGAGTGCCCGCCGCCACACTCAAGCTGCGTCGCCAGCCGGCCATG
GCCCCAGGCGTCACGGGCCTGCGCAACCTGGGCAACACCTGCTACATGAA
CTCCATCCTCCAGGTGCTCAGCCACCTCCAGAAGTTCCGAGAATGTTTCC
TCAACCTTGACCCTTCCAAAACGGAACATCTGTTTCCCAAAGCCACCAAC
GGGAAGACTCAGCTTTCTGGCAAGCCAACCAACAGCTCGGCCACGGAGCT
GTCCTTGAGAAATGACAGGGCCGAGGCATGCGAGCGGGAGGGCTTCTGCT
GGAACGGCAGGGCCTCCATTAGTCGGAGTCTGGAGCTCATCCAGAACAAG
GAGCCGAGTTCAAAGCACATTTCCCTCTGCCGTGAACTGCACACCCTCTT
CCGAGTCATGTGGTCCGGGAAGTGGGCCCTAGTGTCGCCCTTCGCCATGC
TGCACTCAGTGTGGAGCCTGATCCCTGCCTTCCGCGGCTACGACCAACAG
GACGCGCAGGAATTTCTCTGCGAGCTGCTGCACAAGGTGCAGCAGGAACT
CGAGTCTGAGGGCACCACACGCCGGATCCTCATCCCCTTCTCCCAGAGGA
AGCTCACCAAACAGGTCTTAAAGGTGGTGAATACCATATTTCATGGGCAG
CTGCTCAGTCAGGTCACATGTATATCATGCAATTACAAATCCAATACCAT
TGAGCCCTTTTGGGACCTATCCCTGGAATTCCCTGAACGCTATCACTGCA
TAGAAAAGGGGTTTGTCCCTTTGAATCAAACAGAGTGCTTGCTCACTGAG
ATGCTGGCCAAATTCACAGAGACAGAGGCCCTGGAAGGGAGAATCTACGC
TTGTGACCAGTGTAACAGCAAACGACGAAAATCCAATCCCAAACCCCTTG
TTCTGAGTGAAGCTAGAAAGCAGTTAATGATCTACAGACTACCTCAGGTT
CTCCGGCTGCACCTTAAAAGATTCAGGTGGTCTGGCCGTAATCATCGAGA
GAAGATTGGGGTCCATGTCGTCTTTGACCAGGTATTAACCATGGAACCTT
ACTGCTGCAGGGACATGCTCTCCTCTCTTGACAAAGAGACCTTTGCCTAT
GATCTCTCCGCAGTGGTCATGCATCACGGGAAAGGGTTTGGCTCAGGACA
CTACACAGCCTATTGCTACAACACAGAGGGAGGTGCGTGCGCTTTACTCT
GTGGGGTGGGGGACACGGAAAGGGGTTGAGATCT
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDRCKHVGRLRLAQDHSIL
NPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEV
RDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRS
MASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKL
EQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDA
GPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQ
VLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRN
DRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMW
SGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEG
TTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFW
DLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQC
NSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGV
HVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAY
CYNTEGGACALLCGVGDTERG*


Vector Type
Baculovirus
Antibiotic
Amp
Comments
Baculovirus construct to express GST N terminal fusion

Estimated MW of expressed protein : 100276 Daltons
100042 R 1000 1001 1047 19007
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