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Plasmid
pGEX6P-3-(mouse) ZNRF2
Parent Plasmid
pGEX6P3
DU Number
DU36367
Genbank
NP_954594.1 GI:40255287
Species
Mouse
Sequence of Insert
View Sequence
GGATCCATGGGCGCAAAACAGAGCGGACCAGCAGCTAATGGACGCACACG
CGCATATAGCGGCAGCGATCTGCCGAGCGGCACAGGTTCCGGAGGAGGCG
GAGCAGATGGAGCTCGCGCAGCTCGCTTTGCTGCTCCAGTGAGCGGCGCA
CAGCAGCCGTCCGCAAGCGCTGGAGCAGCCGCTGCTGCAGCCGCAGCTGC
TAGCGCACCAGCTGCACCACGCTCCCGCAGCCTGGGCGGAGCAGTGGGAA
GCGCTTCCGGAGGCCGCGCAGCCCAGTCCGCATTCAGCATTCCGTCCGCA
GGCGGAGGCGGAGGACCATATGGCAGCCAGGATAGCGTGCACAGCAGCCC
AGAAGATAGCGTGGGAGCTCGCGATCGCGATCGCCCGGCTGGCGGAGGCC
CAGGAGGACCACGCCTGGTGATTGGCAGCCTGCCGGCACACCTGAGCCCG
CATCTGTTTGGCGGATTCAAATGCCCAGTGTGCAGCAAATTTGTGCCGAG
CGATGAAATGGATCTGCACCTGGTGATGTGCCTGACCAAACCACGCATCA
CCTACAACGAAGATGTGCTGAGCAAAGATACCGGAGAGTGCGCCATCTGC
CTGGAAGAGCTGCAGCAGGGCGATACCATTGCCCGCCTGCCGTGCCTGTG
CATCTATCACAAAGGATGCATCGATGAATGGTTTGAAGTGAACCGCAGCT
GCCCAGAACATCCAAGCGATTAAGCGGCCGC
CGCATATAGCGGCAGCGATCTGCCGAGCGGCACAGGTTCCGGAGGAGGCG
GAGCAGATGGAGCTCGCGCAGCTCGCTTTGCTGCTCCAGTGAGCGGCGCA
CAGCAGCCGTCCGCAAGCGCTGGAGCAGCCGCTGCTGCAGCCGCAGCTGC
TAGCGCACCAGCTGCACCACGCTCCCGCAGCCTGGGCGGAGCAGTGGGAA
GCGCTTCCGGAGGCCGCGCAGCCCAGTCCGCATTCAGCATTCCGTCCGCA
GGCGGAGGCGGAGGACCATATGGCAGCCAGGATAGCGTGCACAGCAGCCC
AGAAGATAGCGTGGGAGCTCGCGATCGCGATCGCCCGGCTGGCGGAGGCC
CAGGAGGACCACGCCTGGTGATTGGCAGCCTGCCGGCACACCTGAGCCCG
CATCTGTTTGGCGGATTCAAATGCCCAGTGTGCAGCAAATTTGTGCCGAG
CGATGAAATGGATCTGCACCTGGTGATGTGCCTGACCAAACCACGCATCA
CCTACAACGAAGATGTGCTGAGCAAAGATACCGGAGAGTGCGCCATCTGC
CTGGAAGAGCTGCAGCAGGGCGATACCATTGCCCGCCTGCCGTGCCTGTG
CATCTATCACAAAGGATGCATCGATGAATGGTTTGAAGTGAACCGCAGCT
GCCCAGAACATCCAAGCGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGAKQSGPAANGRTRAYSG
SDLPSGTGSGGGGADGARAARFAAPVSGAQQPSASAGAAAAAAAAASAPA
APRSRSLGGAVGSASGGRAAQSAFSIPSAGGGGGPYGSQDSVHSSPEDSV
GARDRDRPAGGGPGGPRLVIGSLPAHLSPHLFGGFKCPVCSKFVPSDEMD
LHLVMCLTKPRITYNEDVLSKDTGECAICLEELQQGDTIARLPCLCIYHK
GCIDEWFEVNRSCPEHPSD*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGAKQSGPAANGRTRAYSG
SDLPSGTGSGGGGADGARAARFAAPVSGAQQPSASAGAAAAAAAAASAPA
APRSRSLGGAVGSASGGRAAQSAFSIPSAGGGGGPYGSQDSVHSSPEDSV
GARDRDRPAGGGPGGPRLVIGSLPAHLSPHLFGGFKCPVCSKFVPSDEMD
LHLVMCLTKPRITYNEDVLSKDTGECAICLEELQQGDTIARLPCLCIYHK
GCIDEWFEVNRSCPEHPSD*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Bacterial construct to express GST N terminal fusion.
Estimated MW of expressed protein : 50526 Daltons
All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.