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Nemo (mouse)

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Plasmid
pBS-CMV-Puro/Neo-IRES/TK Nemo targeting vector (SD/arm2/Mut/arm1)
Parent Plasmid
pGEX6P3
DU Number
DU11607
Genbank
NM_003071.3*
Species
Human
Sequence of Insert
GTCGACATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCA
GACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAA
CTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTT
CTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGG
TCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAA
TGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATT
AAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCT
TGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAG
GGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATG
ACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAA
GAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATT
TGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCA
GTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGA
CAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAAC
CAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTA
GCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGA
ACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGG
ACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTG
GGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGG
CAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAAT
ATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAA
AAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAG
TATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTA
GCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGT
GATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAA
ACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGGATCTTCTA
AGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCT
ACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTC
AAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTC
CGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAA
TCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAG
AGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATA
TTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATA
AGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAA
TGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGG
TTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTT
CTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAG
AACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTT
TACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAA
ATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCA
GCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAA
ATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTG
GCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTG
TTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAG
GAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAG
TTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTC
TTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCT
GTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTA
TGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGA
ATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTT
CAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAAT
CCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTT
AATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATG
GTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAAC
ACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGG
AGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAG
CCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTT
GGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGT
TGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAG
GAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAA
ATTAATGAAATCAGAACATTAATTGACTTACACCATCACCATCACCATTAAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMSWMFKRDPVWKY
LQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSL
RGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKK
ELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQ
LKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTE
FDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPF
WEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFH
DGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQ
PSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMK
GKLKNVQSETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVE
GSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDR
IREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRN
PNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWW
HRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVF
IQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQ
ICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICL
DSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPP
EELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFL
SLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKA
GGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKD
SVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDLHHHHHH*
Vector Type
Other
Antibiotic
Amp
Comments
Bacterial construct to express GST N terminal fusion and C-ter 6His.
Sequence differences compared with NM_003071.3 CDS:
g909a (sil T303)
delta agc nt1284-1286 (delta residue A429*)
*derives from EST -also deleted in some published seqs.

Estimated MW of expressed protein : 142169 Daltons
100042 R 1000 1001 1047 19007
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