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Pellino2

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Plasmid
pEBG6P-Pellino2
Parent Plasmid
pEBG6P
Expressed
GST-Pellino 2
DU Number
DU12085
Genbank
AAH09476.1
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGA
GCCAGTGAAATACGGGGAGCTGGTGGTGCTCGGGTACAATGGTGCTTTAC
CCAATGGAGATAGAGGACGGAGGAAAAGTAGATTTGCCCTCTACAAGCGG
CCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCATGTGATATCCACGCC
CCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCCTACA
CTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGAT
ACGGATATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGT
TGTCACAGACACGATTTCTGGCAGCCAGAACACGGACGAAGCCCAGATCA
CACAGAGCACCATATCCAGGTTCGCCTGCAGGATCGTGTGCGACAGGAAT
GAACCTTACACAGCACGGATATTCGCCGCCGGATTTGACTCTTCCAAAAA
CATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGCCACA
TGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGC
TTCACCGAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGG
AGATGTGTACACCTTGCGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGC
TGGTGGAAAGTGAGACCAACGTCCTGCAGGACGGCTCCCTCATTGACCTG
TGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGGCTTTTTCATACTCC
AACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCCCGGC
CTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGG
AAAGAGGTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCA
CGTGCACGGGTACCACAACTGGGGCCATCGGAGTGACACGGAGGCCAACG
AGAGGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTATGTGCCTCTCTGG
CTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGACCGCCAACTCATGC
TTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATACTGGT
CTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCT
TTCTGTGCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTT
CCAAGGTCCAATTGACTGAGCGGCCGC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMFSPGQEEHCAPN
KEPVKYGELVVLGYNGALPNGDRGRRKSRFALYKRPKANGVKPSTVHVIS
TPQASKAISCKGQHSISYTLSRNQTVVVEYTHDKDTDMFQVGRSTESPID
FVVTDTISGSQNTDEAQITQSTISRFACRIVCDRNEPYTARIFAAGFDSS
KNIFLGEKAAKWKNPDGHMDGLTTNGVLVMHPRGGFTEESQPGVWREISV
CGDVYTLRETRSAQQRGKLVESETNVLQDGSLIDLCGATLLWRTADGLFH
TPTQKHIEALRQEINAARPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSC
GHVHGYHNWGHRSDTEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPT
HAFTPCGHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKL
IFQGPID*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter GST for mammalian expression.

Estimated MW of expressed protein : 73850 Daltons
100042 R 1000 1001 1047 19007
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