Pellino1 | Ubiquitylation Resource

Plasmid

pEBG6P-Pellino1 F11S

Parent Plasmid

pEBG6P

Expressed

GST Pellino1 F11S

DU Number

DU12485

Genbank

Q96FA3

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGTTTTCTCCTGATCAAGAAAATCATCCATCTAAAGCACCAGT
AAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATG
GCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAG
GCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGC
TGCAAAGGCAATAAGCAACAAAGACCAGCATAGCATATCATATACTTTGT
CTCGGGCCCAGACTGTGGTGGTTGAATATACTCATGACAGCAACACAGAT
ATGTTTCAAATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAAC
TGACACGGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAA
GCACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCC
TTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTT
TCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATG
GCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACA
GAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGT
ATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGG
AAATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGT
GCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGT
GAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGT
GCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGAC
GTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACA
TGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTG
AATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGA
TGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAG
CCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGA
TCCCACTTCCCCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGT
GCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGG
ACCTCTAGACTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMFSPDQENHPSKA
PVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTP
QAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFV
VTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKN
IFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCG
NVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTP
TVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGH
VHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHA
FSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIF
QGPLD*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression.

Estimated MW of expressed protein : 73701 Daltons

100042 R 1000 1001 1047 19007