NEMO | Ubiquitylation Resource

Plasmid

pcDNA5-FRT/TO-GFP-TAP Nemo

Parent Plasmid

pcDNA5 FRT/TO GFPTAP

Expressed

GFP TAP Nemo

DU Number

DU12387

Genbank

AAH50612

Tag

GFP

TAP

Species

Human

Sequence of Insert

View Sequence

GGATCCAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCC
CAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTC
TGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAG
ACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCG
GCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCC
AAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAG
GCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAG
GCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGC
AGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTG
CTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGA
ATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGC
AGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTG
GACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGA
GCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGG
CCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTG
GTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCA
GCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATA
AGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCG
GTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAG
GCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGC
TGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAG
TCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGC
CCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCA
GCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAG
TGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGA
GTGCATTGAGTAGGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSNRH
LWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRC
LEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLV
ERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQ
ESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRM
QGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSER
KRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQ
ADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIE
DMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQA
PDMDTLQIHVMECIE*

Vector Type

Mammalian

Antibiotic

Amp

Comments

GFP-TAP mammalian construct.

Estimated MW of expressed protein : 98147 Daltons

100042 R 1000 1001 1047 19007