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Nemo

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Plasmid
pCMV5 Nemo FLAG
Parent Plasmid
pCMV5
Expressed
Nemo FLAG
DU Number
DU12932
Genbank
AAH50612
Tag
FLAG
Species
Human
Sequence of Insert
GAATTCGCCCTTGCGGATCCGCCACCATGAATAGGCACCTCTGGAAGAGC
CAACTGTGTGAGATGGTGCAGCCCAGTGGTGGCCCGGCAGCAGATCAGGA
CGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGC
CTTCAGAACAGGGCGCTCCTGAGACCCTCCAGCGCTGCCTGGAGGAGAAT
CAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCG
CTGCGAGGAGCTTCTGCATTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGT
TCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGC
CTGGAGAAGCTCGATCTGAAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGT
GGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTG
TGAAAGCCCAGGTGACGTCCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGT
CGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCG
GGCGGCCAGCGAGCAGGCGCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGC
AGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGC
GTGGAGGCCGCGCTCCGCATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAG
GAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACG
ACAACCACATCAAGAGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATG
CAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGT
GGCCAAACAGGAGGTGATCGATAAGCTGAAGGAGGAGGCCGAGCAGCACA
AGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTAC
AAGGCGGACTTCCAGGCTGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAA
GAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCA
AACTGAAGGCCAGCTGTCAGGAGTCGGCCAGGATCGAGGACATGAGGAAG
CGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCT
CTCCTCTCCCCTGGCCCTGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGC
CACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGAC
ACCCTGCAGATACATGTCATGGAGTGCATTGAGGACTACAAGGACGATGA
CGATAAGTAAGCGGCCGCCCAAGGGCGAATTC
Amino Acid Sequence
MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPET
LQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEA
RKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLL
GELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVD
QLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVV
GSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPV
LKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQES
ARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKC
QYQAPDMDTLQIHVMECIEDYKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
C ter FLAG for mammalian expression.

Estimated MW of expressed protein : 49190 Daltons
100042 R 1000 1001 1047 19007
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