A20 (mouse)

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Plasmid
pFBHTb A20 (mouse)
Parent Plasmid
pFastBac HTb
Expressed
6His A20 (mouse)
DU Number
DU15700
Genbank
AAH60221
Tag
6His
Species
Mouse
Sequence of Insert
GGATCCATGGCTGAACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATAT
GCGGAAAGCTGTGAAGATACGAGAGAGAACCCCAGAAGACATTTTCAAAC
CTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTG
GAGATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCA
CAAAGCACTTATTGACAGAAGTGTCCAGGCTTCCCTGGAAAGCCAGAAGA
AGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAAAACCAAT
GGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGT
TCAGGATACTGACCTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGG
AGACAGACACTCGGAACTTTAAATTCCGCTGGCAGCTGGAATCTCTGAAA
TCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAA
TGACGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAG
CCCGAAGTGGACTTCAGTACAATTCCCTGGAAGAAATCCACATATTTGTC
CTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACAAAATGCT
AAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGA
TTTATCTGCCTCTTCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATC
GTCCTAGGCTATGACAGCCAGCACTTTGTACCCCTGGTGACCCTGAAGGA
CAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTA
GGTTTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATG
AAGGAAAAGCTTCTAAAGGAGTACTTGATAGTGATGGAGATCCCTGTGCA
AGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAATTGGATG
AAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTT
GTTCAGCACGAATACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAG
AGCGGCACATGCGCAGAACCCCTTGGAGCCTTCCACACCCCAGCTATCAC
TCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTG
AACACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCA
GAGCAAGCTCCCAAAGCTGAACTCGAAGCTAGGCCCTGAAGGACTCCCAG
GCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGCTGGAGGA
CCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGAC
CACTGCAATGAAGTGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGC
AGCATAATGGATTCTGTGAGCGTTGCCACGCCCGGCAGATTAATGCCAGC
CACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCG
GACCTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGC
CCAGCTCCAGCCTCACTTCCAGTATCCCTGCCTCCTGTCACCAACGCTCC
AAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACTCTTGCCA
CCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGA
CTCCAGGAGACAGAGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATG
TATTTTGGGACTCCAGAAAACAAGGGCTTTTGCACTCTATGTTTCATCGA
ATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCC
CGGCCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGC
ACACTCGGAAGCACCATGTTTGAAGGGTACTGTCAGAAGTGTTTCATCGA
AGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAACAGCTGA
GATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGG
CTGAAATGTGCCCGGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGA
GGAACTCTGTATGGAGTGCCAGCACCTAAGCCAACGAGTAGGTTCTGTGG
CCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGG
GCCCCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAA
TGAGTGCTACCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSMAEQLLPQALYLSNMRKAVKIR
ERTPEDIFKPTNGIIYHFKTMHRYTLEMFRTCQFCPQFREIIHKALIDRS
VQASLESQKKLNWCREVRKLVALKTNGDGNCLMHAACQYMWGVQDTDLVL
RKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWNDEWDNLV
KMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSLESGS
NFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRA
VPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTT
HLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNP
LEPSTPQLSLMDIKCETPNCPFFMSVNTQPLFHECSERRQKNQSKLPKLN
SKLGPEGLPGVGLGSSNWSPEETAGGPHSAPPTAPSLFLFSETTAMKCRS
PGCPFTLNVQHNGFCERCHARQINASHTADPGKCQACLQDVTRTFNGICS
TCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHRTGNVSP
SGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQS
VTASEKAGSPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFH
EARRTEEQLRSSQHRDMPRTTQVASRLKCARASCKNILACRSEELCMECQ
HLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYCNECYQFKQ
MYG*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
N terminal 6His for baculovirus expression.

Estimated MW of expressed protein : 91066 Daltons
100042 R 1000 1001 1047 19007
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