NEDD8

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Plasmid
pMalP NEDD8 1-76
Parent Plasmid
pMAL6P
Expressed
MBP NEDD8 1-76
DU Number
DU32136
Genbank
Q15843
Tag
MBP
Species
Human
Sequence of Insert
GGATCCATGCTAATTAAAGTGAAGACGCTGACCGGAAAGGAGATTGAGAT
TGACATTGAACCTACAGACAAGGTGGAGCGAATCAAGGAGCGTGTGGAGG
AGAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCATCTACAGTGGCAAG
CAGATGAATGATGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGGTTC
AGTCCTTCACCTGGTGTTGGCTCTGAGAGGAGGATAAGGTCTTAGGCAGT
GAGCGGCCGC
Amino Acid Sequence
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMLIKVKTLTGKEIEIDIEPTDK
VERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLA
LRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter MBP for bacterial expression

Estimated MW of expressed protein : 55297 Daltons
100042 R 1000 1001 1047 19007
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